Abstract
The pyruvate dehydrogenase complex of the adult parasitic nematode Ascaris suum functions in the reducing environment present in their anaerobic mitochondria. These organelles use fumarate and enoyl CoAs as terminal electron acceptors instead of oxygen. A λgt11 cDNA library was constructed from RNA isolated from adult ascarid muscle. Partial clones for the pyruvate dehydrogenase α subunit were isolated by screening the λgt11 library with a specific antiserum. Full-length clones (type I) were identified in a cDNA library prepared from RNA isolated from early embryos. During the hybridization screening, a second type of cDNA clone (type II) was identified. The nucleotide sequences of both clones are presented. The predicted amino acid sequences of the mature proteins are 91% identical to one another and about 55% identical to the predicted sequences of the α subunit of human pyruvate dehydrogenase. Northern blots were used to examine the expression of both mRNAs in various larval stages and in tissues of the adult. Type I sequences are found mainly in adult muscle. Type II sequences are abundant in third-stage larvae as well as in adult muscle.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 37-47 |
| Number of pages | 11 |
| Journal | Molecular and Biochemical Parasitology |
| Volume | 51 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1992 |
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Keywords
- Ascaris suum
- Molecular cloning
- Pyruvate dehydrogenase complex
ASJC Scopus subject areas
- Molecular Biology
- Parasitology
Cite this
Characterization of cDNA clones for the alpha subunit of pyruvate dehydrogenase from Ascaris suum. / Johnson, Keith R; Komuniecki, Richard; Sun, Yinghao; Wheelock, Margaret J.
In: Molecular and Biochemical Parasitology, Vol. 51, No. 1, 01.01.1992, p. 37-47.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of cDNA clones for the alpha subunit of pyruvate dehydrogenase from Ascaris suum
AU - Johnson, Keith R
AU - Komuniecki, Richard
AU - Sun, Yinghao
AU - Wheelock, Margaret J.
PY - 1992/1/1
Y1 - 1992/1/1
N2 - The pyruvate dehydrogenase complex of the adult parasitic nematode Ascaris suum functions in the reducing environment present in their anaerobic mitochondria. These organelles use fumarate and enoyl CoAs as terminal electron acceptors instead of oxygen. A λgt11 cDNA library was constructed from RNA isolated from adult ascarid muscle. Partial clones for the pyruvate dehydrogenase α subunit were isolated by screening the λgt11 library with a specific antiserum. Full-length clones (type I) were identified in a cDNA library prepared from RNA isolated from early embryos. During the hybridization screening, a second type of cDNA clone (type II) was identified. The nucleotide sequences of both clones are presented. The predicted amino acid sequences of the mature proteins are 91% identical to one another and about 55% identical to the predicted sequences of the α subunit of human pyruvate dehydrogenase. Northern blots were used to examine the expression of both mRNAs in various larval stages and in tissues of the adult. Type I sequences are found mainly in adult muscle. Type II sequences are abundant in third-stage larvae as well as in adult muscle.
AB - The pyruvate dehydrogenase complex of the adult parasitic nematode Ascaris suum functions in the reducing environment present in their anaerobic mitochondria. These organelles use fumarate and enoyl CoAs as terminal electron acceptors instead of oxygen. A λgt11 cDNA library was constructed from RNA isolated from adult ascarid muscle. Partial clones for the pyruvate dehydrogenase α subunit were isolated by screening the λgt11 library with a specific antiserum. Full-length clones (type I) were identified in a cDNA library prepared from RNA isolated from early embryos. During the hybridization screening, a second type of cDNA clone (type II) was identified. The nucleotide sequences of both clones are presented. The predicted amino acid sequences of the mature proteins are 91% identical to one another and about 55% identical to the predicted sequences of the α subunit of human pyruvate dehydrogenase. Northern blots were used to examine the expression of both mRNAs in various larval stages and in tissues of the adult. Type I sequences are found mainly in adult muscle. Type II sequences are abundant in third-stage larvae as well as in adult muscle.
KW - Ascaris suum
KW - Molecular cloning
KW - Pyruvate dehydrogenase complex
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U2 - 10.1016/0166-6851(92)90198-S
DO - 10.1016/0166-6851(92)90198-S
M3 - Article
VL - 51
SP - 37
EP - 47
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
SN - 0166-6851
IS - 1
ER -