Characterization of cDNA clones for the alpha subunit of pyruvate dehydrogenase from Ascaris suum

Keith R Johnson, Richard Komuniecki, Yinghao Sun, Margaret J. Wheelock

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The pyruvate dehydrogenase complex of the adult parasitic nematode Ascaris suum functions in the reducing environment present in their anaerobic mitochondria. These organelles use fumarate and enoyl CoAs as terminal electron acceptors instead of oxygen. A λgt11 cDNA library was constructed from RNA isolated from adult ascarid muscle. Partial clones for the pyruvate dehydrogenase α subunit were isolated by screening the λgt11 library with a specific antiserum. Full-length clones (type I) were identified in a cDNA library prepared from RNA isolated from early embryos. During the hybridization screening, a second type of cDNA clone (type II) was identified. The nucleotide sequences of both clones are presented. The predicted amino acid sequences of the mature proteins are 91% identical to one another and about 55% identical to the predicted sequences of the α subunit of human pyruvate dehydrogenase. Northern blots were used to examine the expression of both mRNAs in various larval stages and in tissues of the adult. Type I sequences are found mainly in adult muscle. Type II sequences are abundant in third-stage larvae as well as in adult muscle.

Original languageEnglish (US)
Pages (from-to)37-47
Number of pages11
JournalMolecular and Biochemical Parasitology
Volume51
Issue number1
DOIs
StatePublished - Jan 1 1992

Fingerprint

Ascaris suum
Pyruvic Acid
Oxidoreductases
Complementary DNA
Clone Cells
Gene Library
Muscles
RNA
Pyruvate Dehydrogenase Complex
Fumarates
Northern Blotting
Organelles
Libraries
Larva
Immune Sera
Amino Acid Sequence
Mitochondria
Embryonic Structures
Electrons
Oxygen

Keywords

  • Ascaris suum
  • Molecular cloning
  • Pyruvate dehydrogenase complex

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

Cite this

Characterization of cDNA clones for the alpha subunit of pyruvate dehydrogenase from Ascaris suum. / Johnson, Keith R; Komuniecki, Richard; Sun, Yinghao; Wheelock, Margaret J.

In: Molecular and Biochemical Parasitology, Vol. 51, No. 1, 01.01.1992, p. 37-47.

Research output: Contribution to journalArticle

Johnson, Keith R ; Komuniecki, Richard ; Sun, Yinghao ; Wheelock, Margaret J. / Characterization of cDNA clones for the alpha subunit of pyruvate dehydrogenase from Ascaris suum. In: Molecular and Biochemical Parasitology. 1992 ; Vol. 51, No. 1. pp. 37-47.
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