Characterization of cadherin-24, a novel alternatively spliced type II cadherin

Bryan J. Katafiasz, Marvin T. Nieman, Margaret J. Wheelock, Keith R Johnson

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Cadherins comprise a superfamily of calcium-dependent cell-cell adhesion molecules. Within the superfamily are six subfamilies including type I and type II cadherins. Both type I and type II cadherins are composed of five extracellular repeat domains with conserved calcium-binding motifs, a single pass transmembrane domain, and a highly conserved cytoplasmic domain that interacts with β-catenin and p120 catenin. In this study, we describe a novel cadherin, cadherin-24. It is a type II cadherin with a 781-codon open reading frame, which encodes a type II cadherin protein complete with five extracellular repeats containing calcium-binding motifs, a transmembrane domain, and a conserved cytoplasmic domain. Cadherin-24 has the unusual feature of being alternatively spliced in extracellular repeat 4. This alternative exon encodes 38 in-frame amino acids, resulting in an 819-amino-acid protein. Sequence analysis suggests the presence of β-catenin and p120 catenin-binding sequences, and immunoprecipitation experiments confirm the ability of both forms of the novel cadherin to associate with α-catenin, β-catenin, and p120 catenin. In addition, aggregation assays show that both forms of cadherin-24 mediate strong cell-cell adhesion.

Original languageEnglish (US)
Pages (from-to)27513-27519
Number of pages7
JournalJournal of Biological Chemistry
Volume278
Issue number30
DOIs
StatePublished - Jul 25 2003

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Cadherins
Catenins
Calcium
Amino Acids
Cell adhesion
Cell Adhesion Molecules
Immunoprecipitation
Cell Adhesion
Codon
Open Reading Frames
Sequence Analysis
Exons
Assays
Proteins
Agglomeration

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Characterization of cadherin-24, a novel alternatively spliced type II cadherin. / Katafiasz, Bryan J.; Nieman, Marvin T.; Wheelock, Margaret J.; Johnson, Keith R.

In: Journal of Biological Chemistry, Vol. 278, No. 30, 25.07.2003, p. 27513-27519.

Research output: Contribution to journalArticle

Katafiasz, Bryan J. ; Nieman, Marvin T. ; Wheelock, Margaret J. ; Johnson, Keith R. / Characterization of cadherin-24, a novel alternatively spliced type II cadherin. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 30. pp. 27513-27519.
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