Characterization of barnacle (Balanus eburneus and B. cenatus) adhesive proteins

M. J. Naldrett, D. L. Kaplan

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Cements from two species of barnacles, Bulanus eburneus Gould and Balanus crenatus Bruguiere, were analyzed to identify the number and nature of proteins present. B. crenatus cement was composed mainly of a small peptide cross-linked into aggregates ranging in size from ~3 kD to 40 50 kD. These aggregates could be reduced with 2-mercaptoethanol (2-ME) in the presence of sodium dodecyl sulfate (SDS) but only after incubation at 40 °C for 12 to 24 h and only when the cement was recently formed. Reductive alkylation of cysteine residues with 4-vinylpyridine produced a water-soluble peptide of less than 5 kD. By comparison, the cement of B. eburneus could be dissolved only partially in SDS and 2-ME when heated at 100 °C for 10 min. Five major proteins were identified by SDS-PAGE: 7, 22, 36 and 58 kD bands for which N-terminal sequence and amino acid compositions are presented; and a 52 kD band for which sequence data are given. A minor protein band of ~80 kD has the same N-terminus as the 36 kD band. CNBr digests of individual proteins produced peptides for which sequence and composition data are also presented. The study was conducted during 1993 to 1995. In general, the proteins identified from B. crenatus cement are similar to those characterized from B. eburneus, and they are different in composition and sequence than those previously reported from Mytilus edulis.

Original languageEnglish (US)
Pages (from-to)629-635
Number of pages7
JournalMarine Biology
Volume127
Issue number4
DOIs
StatePublished - Mar 1 1997

Fingerprint

Balanus
Cirripedia
cement
adhesives
sodium dodecyl sulfate
protein
peptide
sodium
protein aggregates
peptides
sulfate
proteins
alkylation
Mytilus edulis
amino acid composition
polyacrylamide gel electrophoresis
cysteine
amino acid
incubation
adhesive

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science
  • Ecology

Cite this

Characterization of barnacle (Balanus eburneus and B. cenatus) adhesive proteins. / Naldrett, M. J.; Kaplan, D. L.

In: Marine Biology, Vol. 127, No. 4, 01.03.1997, p. 629-635.

Research output: Contribution to journalArticle

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