Characterization of antibody response to porcine reproductive and respiratory syndrome virus ORF5 product following infection and evaluation of its diagnostic use in pigs

Jimmy Kwang, Simon Yang, Fernando A. Osorio, Steve Christian, J. Galeota Wheeler, Kelly M. Lager, Sharon Low, Leo Chang, Alan R Doster, Amy White, Ching Ching Wu

Research output: Contribution to journalReview article

3 Citations (Scopus)

Abstract

The sensitivity and specificity of recombinant open reading frame 5 products used in the Western blotting assay for confirmation of porcine reproductive and respiratory syndrome virus (PRRSV) serologic status were evaluated. The recombinant antigen-based assays were specifically compared with a commercial enzyme-linked immunosorbent assay (ELISA) for PRRSV antibodies using 1) PRRSV antibody-negative reference sera (n = 30), 2) naturally infected pig sera (n = 40), 3) sequential sera obtained from 24 experimentally infected pigs, and 4) sera submitted to 3 state diagnostic laboratories (n = 200). The recombinant antigen assay yielded an average increased sensitivity of 10% over the commercial PRRSV ELISA. The negative controls (group 1 sera) showed no difference between the 2 assays. This comparison confirmed that the recombinant antigen-specific assay was more sensitive than the commercial ELISA and is well suited for routine confirmation of the presence of PRRSV antibodies.

Original languageEnglish (US)
Pages (from-to)391-395
Number of pages5
JournalJournal of Veterinary Diagnostic Investigation
Volume11
Issue number5
DOIs
StatePublished - Sep 1999

Fingerprint

Porcine respiratory and reproductive syndrome virus
Porcine reproductive and respiratory syndrome virus
Antibody Formation
Swine
recombinant antigens
swine
antibodies
assays
Infection
Serum
infection
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
Antigens
Antibodies
Open Reading Frames
open reading frames
Western blotting
Western Blotting
Sensitivity and Specificity

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Characterization of antibody response to porcine reproductive and respiratory syndrome virus ORF5 product following infection and evaluation of its diagnostic use in pigs. / Kwang, Jimmy; Yang, Simon; Osorio, Fernando A.; Christian, Steve; Wheeler, J. Galeota; Lager, Kelly M.; Low, Sharon; Chang, Leo; Doster, Alan R; White, Amy; Wu, Ching Ching.

In: Journal of Veterinary Diagnostic Investigation, Vol. 11, No. 5, 09.1999, p. 391-395.

Research output: Contribution to journalReview article

Kwang, Jimmy ; Yang, Simon ; Osorio, Fernando A. ; Christian, Steve ; Wheeler, J. Galeota ; Lager, Kelly M. ; Low, Sharon ; Chang, Leo ; Doster, Alan R ; White, Amy ; Wu, Ching Ching. / Characterization of antibody response to porcine reproductive and respiratory syndrome virus ORF5 product following infection and evaluation of its diagnostic use in pigs. In: Journal of Veterinary Diagnostic Investigation. 1999 ; Vol. 11, No. 5. pp. 391-395.
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abstract = "The sensitivity and specificity of recombinant open reading frame 5 products used in the Western blotting assay for confirmation of porcine reproductive and respiratory syndrome virus (PRRSV) serologic status were evaluated. The recombinant antigen-based assays were specifically compared with a commercial enzyme-linked immunosorbent assay (ELISA) for PRRSV antibodies using 1) PRRSV antibody-negative reference sera (n = 30), 2) naturally infected pig sera (n = 40), 3) sequential sera obtained from 24 experimentally infected pigs, and 4) sera submitted to 3 state diagnostic laboratories (n = 200). The recombinant antigen assay yielded an average increased sensitivity of 10{\%} over the commercial PRRSV ELISA. The negative controls (group 1 sera) showed no difference between the 2 assays. This comparison confirmed that the recombinant antigen-specific assay was more sensitive than the commercial ELISA and is well suited for routine confirmation of the presence of PRRSV antibodies.",
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AU - Lager, Kelly M.

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AU - Chang, Leo

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