Characterization of an unusual isoenzyme of N-acetyl-β-D-hexosaminidase from a human colonic carcinoma cell line

P. M. Kimball, M. G. Brattain, W. E. White

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Abstract

A sub-line with increased metastatic ability was previously isolated from an established human colonic carcinoma cell line. The separation and characterization of the isoenzymes of N-acetyl-β-D-hexosaminidase from each cell line are reported. The parental cell line contained A and B isoenzymes. The sub-line lacked the A-isoenzyme activity and contained an atypical B isoenzyme that was thermolabile, susceptible to alkylation and of lower molecular weight.

Original languageEnglish (US)
Pages (from-to)109-113
Number of pages5
JournalBiochemical Journal
Volume193
Issue number1
DOIs
Publication statusPublished - Jan 1 1981

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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