Characterization of an unusual isoenzyme of N-acetyl-β-D-hexosaminidase from a human colonic carcinoma cell line

P. M. Kimball, Michael G Brattain, W. E. White

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A sub-line with increased metastatic ability was previously isolated from an established human colonic carcinoma cell line. The separation and characterization of the isoenzymes of N-acetyl-β-D-hexosaminidase from each cell line are reported. The parental cell line contained A and B isoenzymes. The sub-line lacked the A-isoenzyme activity and contained an atypical B isoenzyme that was thermolabile, susceptible to alkylation and of lower molecular weight.

Original languageEnglish (US)
Pages (from-to)109-113
Number of pages5
JournalBiochemical Journal
Volume193
Issue number1
DOIs
StatePublished - Jan 1 1981

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Hexosaminidases
Isoenzymes
Cells
Carcinoma
Cell Line
Alkylation
Molecular Weight
Molecular weight

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Characterization of an unusual isoenzyme of N-acetyl-β-D-hexosaminidase from a human colonic carcinoma cell line. / Kimball, P. M.; Brattain, Michael G; White, W. E.

In: Biochemical Journal, Vol. 193, No. 1, 01.01.1981, p. 109-113.

Research output: Contribution to journalArticle

Kimball, P. M. ; Brattain, Michael G ; White, W. E. / Characterization of an unusual isoenzyme of N-acetyl-β-D-hexosaminidase from a human colonic carcinoma cell line. In: Biochemical Journal. 1981 ; Vol. 193, No. 1. pp. 109-113.
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