Characterization of an NBS-LRR resistance gene homologue from soybean

Bangjun Wang, Yongjun Wang, Qiang Wang, Gyangzuo Luo, Zhigang Zhang, Chaoying He, Jie He Si, Jinsong Zhang, Junyi Gai, Shouyi Chen

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Conserved motifs such as the nucleotide-binding site (NBS) were found in many characterized plant disease resistance genes. Based on the NBS domain, resistance gene analogs have been isolated in our previous study and were used as probes to screen a soybean (Glycine max) cDNA library. A full-length cDNA, KR4, was isolated by screening the library and rapid amplification of cDNA ends method. Sequence analysis revealed that the cDNA was 3818 bp in length and the open reading frame coded for a polypeptide of 1211 amino acids with an NBS and five leucine-rich repeats domains, which were identified by Pfam protein analysis. Sequence alignment showed that KR4 was similar to 12 protein of tomato. Southern analysis indicated that the KR4 gene had low copies in soybean genome and it was mapped on the molecular linkage group E. Its expression was also investigated and it was found that KR4 was induced by exogenous salicylic acid and responded upon infection of soybean mosaic virus strain N3.

Original languageEnglish (US)
Pages (from-to)815-822
Number of pages8
JournalJournal of Plant Physiology
Volume161
Issue number7
DOIs
StatePublished - Jan 1 2004
Externally publishedYes

Fingerprint

Soybeans
binding sites
Nucleotides
nucleotides
Binding Sites
soybeans
Complementary DNA
Soybean mosaic virus
Genes
rapid amplification of cDNA ends
genes
sequence alignment
plant diseases and disorders
Plant Diseases
linkage groups
Mosaic Viruses
salicylic acid
cDNA libraries
leucine
Disease Resistance

Keywords

  • Leucine-rich repeat (LRR)
  • Nucleotide-binding site (NBS)
  • Resistance gene homologue
  • Soybean
  • Soybean mosaic virus (SMV)

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Characterization of an NBS-LRR resistance gene homologue from soybean. / Wang, Bangjun; Wang, Yongjun; Wang, Qiang; Luo, Gyangzuo; Zhang, Zhigang; He, Chaoying; Si, Jie He; Zhang, Jinsong; Gai, Junyi; Chen, Shouyi.

In: Journal of Plant Physiology, Vol. 161, No. 7, 01.01.2004, p. 815-822.

Research output: Contribution to journalArticle

Wang, B, Wang, Y, Wang, Q, Luo, G, Zhang, Z, He, C, Si, JH, Zhang, J, Gai, J & Chen, S 2004, 'Characterization of an NBS-LRR resistance gene homologue from soybean', Journal of Plant Physiology, vol. 161, no. 7, pp. 815-822. https://doi.org/10.1016/j.jplph.2004.01.007
Wang, Bangjun ; Wang, Yongjun ; Wang, Qiang ; Luo, Gyangzuo ; Zhang, Zhigang ; He, Chaoying ; Si, Jie He ; Zhang, Jinsong ; Gai, Junyi ; Chen, Shouyi. / Characterization of an NBS-LRR resistance gene homologue from soybean. In: Journal of Plant Physiology. 2004 ; Vol. 161, No. 7. pp. 815-822.
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AU - He, Chaoying

AU - Si, Jie He

AU - Zhang, Jinsong

AU - Gai, Junyi

AU - Chen, Shouyi

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AB - Conserved motifs such as the nucleotide-binding site (NBS) were found in many characterized plant disease resistance genes. Based on the NBS domain, resistance gene analogs have been isolated in our previous study and were used as probes to screen a soybean (Glycine max) cDNA library. A full-length cDNA, KR4, was isolated by screening the library and rapid amplification of cDNA ends method. Sequence analysis revealed that the cDNA was 3818 bp in length and the open reading frame coded for a polypeptide of 1211 amino acids with an NBS and five leucine-rich repeats domains, which were identified by Pfam protein analysis. Sequence alignment showed that KR4 was similar to 12 protein of tomato. Southern analysis indicated that the KR4 gene had low copies in soybean genome and it was mapped on the molecular linkage group E. Its expression was also investigated and it was found that KR4 was induced by exogenous salicylic acid and responded upon infection of soybean mosaic virus strain N3.

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KW - Nucleotide-binding site (NBS)

KW - Resistance gene homologue

KW - Soybean

KW - Soybean mosaic virus (SMV)

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