Characterization of a serologic marker candidate for development of a live-attenuated DIVA vaccine against porcine reproductive and respiratory syndrome virus

Hiep L Vu, Byungjoon Kwon, Marcelo de Lima, Asit K Pattnaik, Fernando A. Osorio

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

DIVA (differentiating infected from vaccinated animals) vaccines have proven extremely useful for control and eradication of infectious diseases in livestock. We describe here the characterization of a serologic marker epitope, so-called epitope-M201, which can be a potential target for development of a live-attenuated DIVA vaccine against porcine reproductive and respiratory syndrome virus (PRRSV). Epitope-M201 is located at the carboxyl terminus (residues 161-174) of the viral M protein. The epitope is highly immunodominant and well-conserved among type-II PRRSV isolates. Rabbit polyclonal antibodies prepared against this epitope are non-neutralizing; thus, the epitope does not seem to contribute to the protective immunity against PRRSV infection. Importantly, the immunogenicity of epitope-M201 can be disrupted through the introduction of a single amino acid mutation which does not adversely affect the viral replication. All together, our results provide an important starting point for the development of a live-attenuated DIVA vaccine against type-II PRRSV.

Original languageEnglish (US)
Pages (from-to)4330-4337
Number of pages8
JournalVaccine
Volume31
Issue number40
DOIs
StatePublished - Sep 13 2013

Fingerprint

Porcine respiratory and reproductive syndrome virus
Porcine reproductive and respiratory syndrome virus
epitopes
Epitopes
Vaccines
vaccines
animals
Viral Matrix Proteins
Livestock
Virus Diseases
virus replication
polyclonal antibodies
infectious diseases
Communicable Diseases
Immunity
livestock
immunity
rabbits
immune response
Rabbits

Keywords

  • DIVA vaccines
  • M protein
  • PRRSV
  • Serological marker epitope

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Characterization of a serologic marker candidate for development of a live-attenuated DIVA vaccine against porcine reproductive and respiratory syndrome virus. / Vu, Hiep L; Kwon, Byungjoon; de Lima, Marcelo; Pattnaik, Asit K; Osorio, Fernando A.

In: Vaccine, Vol. 31, No. 40, 13.09.2013, p. 4330-4337.

Research output: Contribution to journalArticle

@article{76fcb688fb014325b8047155fa82c68d,
title = "Characterization of a serologic marker candidate for development of a live-attenuated DIVA vaccine against porcine reproductive and respiratory syndrome virus",
abstract = "DIVA (differentiating infected from vaccinated animals) vaccines have proven extremely useful for control and eradication of infectious diseases in livestock. We describe here the characterization of a serologic marker epitope, so-called epitope-M201, which can be a potential target for development of a live-attenuated DIVA vaccine against porcine reproductive and respiratory syndrome virus (PRRSV). Epitope-M201 is located at the carboxyl terminus (residues 161-174) of the viral M protein. The epitope is highly immunodominant and well-conserved among type-II PRRSV isolates. Rabbit polyclonal antibodies prepared against this epitope are non-neutralizing; thus, the epitope does not seem to contribute to the protective immunity against PRRSV infection. Importantly, the immunogenicity of epitope-M201 can be disrupted through the introduction of a single amino acid mutation which does not adversely affect the viral replication. All together, our results provide an important starting point for the development of a live-attenuated DIVA vaccine against type-II PRRSV.",
keywords = "DIVA vaccines, M protein, PRRSV, Serological marker epitope",
author = "Vu, {Hiep L} and Byungjoon Kwon and {de Lima}, Marcelo and Pattnaik, {Asit K} and Osorio, {Fernando A.}",
year = "2013",
month = "9",
day = "13",
doi = "10.1016/j.vaccine.2013.07.020",
language = "English (US)",
volume = "31",
pages = "4330--4337",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "40",

}

TY - JOUR

T1 - Characterization of a serologic marker candidate for development of a live-attenuated DIVA vaccine against porcine reproductive and respiratory syndrome virus

AU - Vu, Hiep L

AU - Kwon, Byungjoon

AU - de Lima, Marcelo

AU - Pattnaik, Asit K

AU - Osorio, Fernando A.

PY - 2013/9/13

Y1 - 2013/9/13

N2 - DIVA (differentiating infected from vaccinated animals) vaccines have proven extremely useful for control and eradication of infectious diseases in livestock. We describe here the characterization of a serologic marker epitope, so-called epitope-M201, which can be a potential target for development of a live-attenuated DIVA vaccine against porcine reproductive and respiratory syndrome virus (PRRSV). Epitope-M201 is located at the carboxyl terminus (residues 161-174) of the viral M protein. The epitope is highly immunodominant and well-conserved among type-II PRRSV isolates. Rabbit polyclonal antibodies prepared against this epitope are non-neutralizing; thus, the epitope does not seem to contribute to the protective immunity against PRRSV infection. Importantly, the immunogenicity of epitope-M201 can be disrupted through the introduction of a single amino acid mutation which does not adversely affect the viral replication. All together, our results provide an important starting point for the development of a live-attenuated DIVA vaccine against type-II PRRSV.

AB - DIVA (differentiating infected from vaccinated animals) vaccines have proven extremely useful for control and eradication of infectious diseases in livestock. We describe here the characterization of a serologic marker epitope, so-called epitope-M201, which can be a potential target for development of a live-attenuated DIVA vaccine against porcine reproductive and respiratory syndrome virus (PRRSV). Epitope-M201 is located at the carboxyl terminus (residues 161-174) of the viral M protein. The epitope is highly immunodominant and well-conserved among type-II PRRSV isolates. Rabbit polyclonal antibodies prepared against this epitope are non-neutralizing; thus, the epitope does not seem to contribute to the protective immunity against PRRSV infection. Importantly, the immunogenicity of epitope-M201 can be disrupted through the introduction of a single amino acid mutation which does not adversely affect the viral replication. All together, our results provide an important starting point for the development of a live-attenuated DIVA vaccine against type-II PRRSV.

KW - DIVA vaccines

KW - M protein

KW - PRRSV

KW - Serological marker epitope

UR - http://www.scopus.com/inward/record.url?scp=84882822947&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84882822947&partnerID=8YFLogxK

U2 - 10.1016/j.vaccine.2013.07.020

DO - 10.1016/j.vaccine.2013.07.020

M3 - Article

VL - 31

SP - 4330

EP - 4337

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 40

ER -