Characterization of a rice gene family encoding root-specific proteins

Yong Xu, Wallace G Buchholz, Richard T. DeRose, Timothy C. Hall

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Two cDNA clones (RCc2 and RCc3) corresponding to mRNAs highly expressed only in root tissues of rice (Oryza sativa L.) seedlings were characterized. Respectively, they encode polypeptides of 146 (14.5 kDa) and 133 amino acids (13.4 kDa) that share high (<70%) sequence similarity with a polypeptide encoded by a cDNA (ZRP3) encoding an mRNA preferentially expressed in young maize roots. Genomic DNA blot analysis revealed that they are members of a small gene family and RCg2, the gene corresponding to RCc2, was isolated. A 1656 bp 5′-upstream sequence of RCg2 was translationally fused to a β-glucuronidase (GUS) reporter gene and stable introduction of the chimeric construct into rice was confirmed by PCR and genomic DNA blot analyses. Histochemical analysis of transgenic rice plants containing the full-length chimeric gene showed high levels of GUS activity in mature cells and the elongation and maturation zones of primary and secondary roots, and in the root caps, but no GUS activity was detected in root meristematic regions. Surprisingly, high GUS activity was also detected in leaves of the same plants. This raises the possibility that the RCg2 5′-upstream element may not be sufficient for the proper spatial control of root specificity in transgenic rice.

Original languageEnglish (US)
Pages (from-to)237-248
Number of pages12
JournalPlant Molecular Biology
Volume27
Issue number2
DOIs
StatePublished - Jan 1 1995

Fingerprint

Glucuronidase
Genes
rice
Proteins
genes
proteins
Complementary DNA
polypeptides
Messenger RNA
Peptides
genetically modified organisms
DNA
genomics
root cap
Plant Leaves
Genetically Modified Plants
Elongation
Seedlings
Reporter Genes
reporter genes

Keywords

  • monocot gene expression
  • root-specific gene expression
  • transgenic rice

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Characterization of a rice gene family encoding root-specific proteins. / Xu, Yong; Buchholz, Wallace G; DeRose, Richard T.; Hall, Timothy C.

In: Plant Molecular Biology, Vol. 27, No. 2, 01.01.1995, p. 237-248.

Research output: Contribution to journalArticle

Xu, Yong ; Buchholz, Wallace G ; DeRose, Richard T. ; Hall, Timothy C. / Characterization of a rice gene family encoding root-specific proteins. In: Plant Molecular Biology. 1995 ; Vol. 27, No. 2. pp. 237-248.
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