Characterization of a Helicobacter hepaticus putA mutant strain in host colonization and oxidative stress

Navasona Krishnan, Alan R Doster, Gerald E. Duhamel, Donald F Becker

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Helicobacter hepaticus is a gram-negative, spiral-shaped microaerophilic bacterium associated with chronic intestinal infection leading to hepatitis and colonic and hepatic carcinomas in susceptible strains of mice. In the closely related human pathogen Helicobacter pylori, L-proline is a preferred respiratory substrate and is found at significantly high levels in the gastric juice of infected patients. A previous study of the proline catabolic PutA flavoenzymes from H. pylori and H. hepaticus revealed that Helicobacter PutA generates reactive oxygen species during proline oxidation by transferring electrons from reduced flavin to molecular oxygen. We further explored the preference for proline as a respiratory substrate and the potential impact of proline metabolism on the redox environment in Helicobacter species during host infection by disrupting the putA gene in H. hepaticus. The resulting putA knockout mutant strain was characterized by oxidative stress analysis and mouse infection studies. The putA mutant strain of H. hepaticus exhibited increased proline levels and resistance to oxidative stress relative to that of the wild-type strain, consistent with proline's role as an antioxidant. The significant increase in stress resistance was attributed to higher proline content, as no upregulation of antioxidant genes was observed for the putA mutant strain. The wild-type and putA mutant H. hepaticus strains displayed similar levels of infection in mice, but in mice challenged with the putA mutant strain, significantly reduced inflammation was observed, suggesting a role for proline metabolism in H. hepaticus pathogenicity in vivo.

Original languageEnglish (US)
Pages (from-to)3037-3044
Number of pages8
JournalInfection and immunity
Volume76
Issue number7
DOIs
StatePublished - Jul 1 2008

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Helicobacter hepaticus
Proline
Oxidative Stress
Helicobacter
Infection
Helicobacter pylori
Antioxidants
Gastric Juice
Hepatitis
Genes
Oxidation-Reduction
Virulence
Reactive Oxygen Species
Up-Regulation

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Characterization of a Helicobacter hepaticus putA mutant strain in host colonization and oxidative stress. / Krishnan, Navasona; Doster, Alan R; Duhamel, Gerald E.; Becker, Donald F.

In: Infection and immunity, Vol. 76, No. 7, 01.07.2008, p. 3037-3044.

Research output: Contribution to journalArticle

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