Characterization of a group D rotavirus.

C. M. Devitt, D. L. Reynolds

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A rotavirus isolated from the intestinal contents of commercial ring-neck pheasant chicks was determined to be a group D rotavirus based on its RNA electropherotype and its serologic relatedness to known turkey group A and D rotaviruses using immune electron microscopy and agar-gel immunodiffusion assays. Further characterization revealed a buoyant density in cesium chloride of 1.347 g/cm3 for double-shelled particles and 1.365 g/cm3 for single-shelled particles. The average size for the pheasant-origin group D rotavirus was determined to be 80 nm for double-shelled particles and 70 nm for single-shelled particles. In contrast, the average size for a turkey group A rotavirus isolate was determined to be 73 nm for double-shelled particles and 60 nm for single-shelled particles. There was no evidence of viral replication by the pheasant-origin group D rotavirus (either cytopathic effect or increase in numbers of viral particles) after six passages in MA104 cells. The hemagglutinating activity of the pheasant origin group D rotavirus was compared with that of a turkey origin group A rotavirus. Differences and similarities were noted depending upon the species of erythrocytes employed and the temperature of incubation.

Original languageEnglish (US)
Pages (from-to)749-755
Number of pages7
JournalAvian diseases
Volume37
Issue number3
DOIs
StatePublished - Jan 1 1993

Fingerprint

Rotavirus D
Rotavirus
pheasants
cesium
cytopathogenicity
virus replication
virion
Gastrointestinal Contents
neck
Replication Origin
Immunodiffusion
electron microscopy
chlorides
erythrocytes
agar
chicks
gels
Virion
RNA
Agar

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Immunology and Microbiology(all)

Cite this

Characterization of a group D rotavirus. / Devitt, C. M.; Reynolds, D. L.

In: Avian diseases, Vol. 37, No. 3, 01.01.1993, p. 749-755.

Research output: Contribution to journalArticle

Devitt, C. M. ; Reynolds, D. L. / Characterization of a group D rotavirus. In: Avian diseases. 1993 ; Vol. 37, No. 3. pp. 749-755.
@article{8d68c8305e484c62bd814a927fe7be28,
title = "Characterization of a group D rotavirus.",
abstract = "A rotavirus isolated from the intestinal contents of commercial ring-neck pheasant chicks was determined to be a group D rotavirus based on its RNA electropherotype and its serologic relatedness to known turkey group A and D rotaviruses using immune electron microscopy and agar-gel immunodiffusion assays. Further characterization revealed a buoyant density in cesium chloride of 1.347 g/cm3 for double-shelled particles and 1.365 g/cm3 for single-shelled particles. The average size for the pheasant-origin group D rotavirus was determined to be 80 nm for double-shelled particles and 70 nm for single-shelled particles. In contrast, the average size for a turkey group A rotavirus isolate was determined to be 73 nm for double-shelled particles and 60 nm for single-shelled particles. There was no evidence of viral replication by the pheasant-origin group D rotavirus (either cytopathic effect or increase in numbers of viral particles) after six passages in MA104 cells. The hemagglutinating activity of the pheasant origin group D rotavirus was compared with that of a turkey origin group A rotavirus. Differences and similarities were noted depending upon the species of erythrocytes employed and the temperature of incubation.",
author = "Devitt, {C. M.} and Reynolds, {D. L.}",
year = "1993",
month = "1",
day = "1",
doi = "10.2307/1592024",
language = "English (US)",
volume = "37",
pages = "749--755",
journal = "Avian Diseases",
issn = "0005-2086",
publisher = "American Association of Avian Pathologists",
number = "3",

}

TY - JOUR

T1 - Characterization of a group D rotavirus.

AU - Devitt, C. M.

AU - Reynolds, D. L.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - A rotavirus isolated from the intestinal contents of commercial ring-neck pheasant chicks was determined to be a group D rotavirus based on its RNA electropherotype and its serologic relatedness to known turkey group A and D rotaviruses using immune electron microscopy and agar-gel immunodiffusion assays. Further characterization revealed a buoyant density in cesium chloride of 1.347 g/cm3 for double-shelled particles and 1.365 g/cm3 for single-shelled particles. The average size for the pheasant-origin group D rotavirus was determined to be 80 nm for double-shelled particles and 70 nm for single-shelled particles. In contrast, the average size for a turkey group A rotavirus isolate was determined to be 73 nm for double-shelled particles and 60 nm for single-shelled particles. There was no evidence of viral replication by the pheasant-origin group D rotavirus (either cytopathic effect or increase in numbers of viral particles) after six passages in MA104 cells. The hemagglutinating activity of the pheasant origin group D rotavirus was compared with that of a turkey origin group A rotavirus. Differences and similarities were noted depending upon the species of erythrocytes employed and the temperature of incubation.

AB - A rotavirus isolated from the intestinal contents of commercial ring-neck pheasant chicks was determined to be a group D rotavirus based on its RNA electropherotype and its serologic relatedness to known turkey group A and D rotaviruses using immune electron microscopy and agar-gel immunodiffusion assays. Further characterization revealed a buoyant density in cesium chloride of 1.347 g/cm3 for double-shelled particles and 1.365 g/cm3 for single-shelled particles. The average size for the pheasant-origin group D rotavirus was determined to be 80 nm for double-shelled particles and 70 nm for single-shelled particles. In contrast, the average size for a turkey group A rotavirus isolate was determined to be 73 nm for double-shelled particles and 60 nm for single-shelled particles. There was no evidence of viral replication by the pheasant-origin group D rotavirus (either cytopathic effect or increase in numbers of viral particles) after six passages in MA104 cells. The hemagglutinating activity of the pheasant origin group D rotavirus was compared with that of a turkey origin group A rotavirus. Differences and similarities were noted depending upon the species of erythrocytes employed and the temperature of incubation.

UR - http://www.scopus.com/inward/record.url?scp=0027622316&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027622316&partnerID=8YFLogxK

U2 - 10.2307/1592024

DO - 10.2307/1592024

M3 - Article

C2 - 8257366

AN - SCOPUS:0027622316

VL - 37

SP - 749

EP - 755

JO - Avian Diseases

JF - Avian Diseases

SN - 0005-2086

IS - 3

ER -