Characterization of a chlorella virus PBCV-1 encoded ribonuclease III

Yuanzheng Zhang, Irina Calin-Jageman, James R. Gurnon, Tae Jin Choi, Byron Adams, Allen W. Nicholson, James L. Van Etten

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Sequence analysis of the 330-kb genome of chlorella virus PBCV-1 revealed an open reading frame, A464R, which encodes a protein with 30-35% amino acid identity to ribonuclease III (RNase III) from many bacteria. The a464r gene was cloned and the protein was expressed in Escherichia coli using the chitin-binding intein system. The recombinant PBCV-1 RNase III cleaves model dsRNA substrates, in a Mg2+-dependent manner, into a defined set of products. The substrate cleavage specificity overlaps, but is nonidentical to that of E. coli RNase III. The a464r gene is expressed very early during PBCV-1 infection, within 5-10 min p.i. The RNase III protein appears at 15 min p.i. and disappears by 120 min p.i. The a464r gene is highly conserved among the chlorella viruses. Phylogenetic analyses indicate that the PBCV enzyme is most closely related to Mycoplasma pneumoniae RNase III.

Original languageEnglish (US)
Pages (from-to)73-83
Number of pages11
JournalVirology
Volume317
Issue number1
DOIs
StatePublished - Dec 5 2003

Fingerprint

Ribonuclease III
Chlorella
Viruses
Inteins
Escherichia coli
Genes
Mycoplasma pneumoniae
Proteins
Chitin
Substrate Specificity
Open Reading Frames
Sequence Analysis
Genome
Bacteria
Amino Acids
Enzymes
Infection

Keywords

  • Chlorella viruses
  • Phycodnaviridae
  • RNase III
  • Ribonuclease
  • dsRNA

ASJC Scopus subject areas

  • Virology

Cite this

Zhang, Y., Calin-Jageman, I., Gurnon, J. R., Choi, T. J., Adams, B., Nicholson, A. W., & Van Etten, J. L. (2003). Characterization of a chlorella virus PBCV-1 encoded ribonuclease III. Virology, 317(1), 73-83. https://doi.org/10.1016/j.virol.2003.08.044

Characterization of a chlorella virus PBCV-1 encoded ribonuclease III. / Zhang, Yuanzheng; Calin-Jageman, Irina; Gurnon, James R.; Choi, Tae Jin; Adams, Byron; Nicholson, Allen W.; Van Etten, James L.

In: Virology, Vol. 317, No. 1, 05.12.2003, p. 73-83.

Research output: Contribution to journalArticle

Zhang, Y, Calin-Jageman, I, Gurnon, JR, Choi, TJ, Adams, B, Nicholson, AW & Van Etten, JL 2003, 'Characterization of a chlorella virus PBCV-1 encoded ribonuclease III', Virology, vol. 317, no. 1, pp. 73-83. https://doi.org/10.1016/j.virol.2003.08.044
Zhang Y, Calin-Jageman I, Gurnon JR, Choi TJ, Adams B, Nicholson AW et al. Characterization of a chlorella virus PBCV-1 encoded ribonuclease III. Virology. 2003 Dec 5;317(1):73-83. https://doi.org/10.1016/j.virol.2003.08.044
Zhang, Yuanzheng ; Calin-Jageman, Irina ; Gurnon, James R. ; Choi, Tae Jin ; Adams, Byron ; Nicholson, Allen W. ; Van Etten, James L. / Characterization of a chlorella virus PBCV-1 encoded ribonuclease III. In: Virology. 2003 ; Vol. 317, No. 1. pp. 73-83.
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