Characterization of 35 microsatellite loci in the Pacific lion-paw scallop (Nodipecten subnodosus) and their cross-species amplification in four other scallops of the Pectinidae family

Ana M. Ibarra, Jessica L. Petersen, Thomas R. Famula, Bernie May

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Four microsatellite-enriched DNA libraries yielded 35 microsatellite loci from 100 primer pairs designed for Pacific lion-paw scallop, Nodipecten subnodosus. The number of alleles ranged from four to 28. Three of the 35 loci were not in Hardy-Weinberg equilibrium and linkage disequilibrium was found for one pair of loci. These microsatellites will be used to analyse the population structure of the species in Mexico's Baja Peninsula to propose management strategies for scallop aquaculture development. Twenty-six primer pairs cross-amplified in Nodipecten nodosus, whereas none (Argopecten ventricosus) or few cross-amplified in the Argopecten species.

Original languageEnglish (US)
Pages (from-to)153-156
Number of pages4
JournalMolecular Ecology Notes
Volume6
Issue number1
DOIs
StatePublished - Mar 1 2006

Fingerprint

Pectinidae
Lions
scallops
Microsatellite Repeats
Amplification
amplification
microsatellite repeats
loci
Argopecten
Argopecten ventricosus
resource development
disequilibrium
population structure
Aquaculture
allele
DNA libraries
Linkage Disequilibrium
linkage disequilibrium
Mexico
Gene Library

Keywords

  • Argopecten
  • Guerrero Negro - Ojo de Liebre lagoon
  • Microsatellite
  • Primers

ASJC Scopus subject areas

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

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title = "Characterization of 35 microsatellite loci in the Pacific lion-paw scallop (Nodipecten subnodosus) and their cross-species amplification in four other scallops of the Pectinidae family",
abstract = "Four microsatellite-enriched DNA libraries yielded 35 microsatellite loci from 100 primer pairs designed for Pacific lion-paw scallop, Nodipecten subnodosus. The number of alleles ranged from four to 28. Three of the 35 loci were not in Hardy-Weinberg equilibrium and linkage disequilibrium was found for one pair of loci. These microsatellites will be used to analyse the population structure of the species in Mexico's Baja Peninsula to propose management strategies for scallop aquaculture development. Twenty-six primer pairs cross-amplified in Nodipecten nodosus, whereas none (Argopecten ventricosus) or few cross-amplified in the Argopecten species.",
keywords = "Argopecten, Guerrero Negro - Ojo de Liebre lagoon, Microsatellite, Primers",
author = "Ibarra, {Ana M.} and Petersen, {Jessica L.} and Famula, {Thomas R.} and Bernie May",
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T1 - Characterization of 35 microsatellite loci in the Pacific lion-paw scallop (Nodipecten subnodosus) and their cross-species amplification in four other scallops of the Pectinidae family

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AU - Petersen, Jessica L.

AU - Famula, Thomas R.

AU - May, Bernie

PY - 2006/3/1

Y1 - 2006/3/1

N2 - Four microsatellite-enriched DNA libraries yielded 35 microsatellite loci from 100 primer pairs designed for Pacific lion-paw scallop, Nodipecten subnodosus. The number of alleles ranged from four to 28. Three of the 35 loci were not in Hardy-Weinberg equilibrium and linkage disequilibrium was found for one pair of loci. These microsatellites will be used to analyse the population structure of the species in Mexico's Baja Peninsula to propose management strategies for scallop aquaculture development. Twenty-six primer pairs cross-amplified in Nodipecten nodosus, whereas none (Argopecten ventricosus) or few cross-amplified in the Argopecten species.

AB - Four microsatellite-enriched DNA libraries yielded 35 microsatellite loci from 100 primer pairs designed for Pacific lion-paw scallop, Nodipecten subnodosus. The number of alleles ranged from four to 28. Three of the 35 loci were not in Hardy-Weinberg equilibrium and linkage disequilibrium was found for one pair of loci. These microsatellites will be used to analyse the population structure of the species in Mexico's Baja Peninsula to propose management strategies for scallop aquaculture development. Twenty-six primer pairs cross-amplified in Nodipecten nodosus, whereas none (Argopecten ventricosus) or few cross-amplified in the Argopecten species.

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