Characterization and regulation of prostaglandin E2 receptors on normal and malignant murine B lymphocytes

Deborah M. Brown, Richard P. Phipps

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Prostaglandin E2 (PGE2) is a pleiotropic lipid molecule synthesized by macrophages, follicular dendritic cells, and fibroblasts, inhabitants of the B cell microenvironment. It is a potent regulator of B lymphocyte functions including activation and immunoglobulin class switching. To understand the effects of PGE2 on B lymphocyte function, the features of the putative PGE2 receptor on cells of the B lineage must be delineated. This receptor has not yet been characterized on B lymphocytes. Murine B cell lymphomas were used as a model to evaluate B lineage PGE2 receptors since they elevate intracellular cAMP in response to PGE2. Scatchard analysis indicates that the PGE2 receptor on both CH31 and CH12 exists in a single high-affinity state, with the CH12 B lymphoma possessing three times more receptors per cell compared to CH31. Interestingly, the PGE2 receptor is subject to regulation as a 20-hr LPS treatment increased PGE2 receptor number by two- to threefold on CH12 and CH31 B cell lymphomas, while dissociation constants remained similar. Finally, Scatchard analysis of normal murine splenic B lymphocytes demonstrates that they also possess high-affinity receptors for PGE2. Treatment of normal B cells with IL-4 increased PGE2 receptor levels fourfold from approximately 50 to 200 sites/cell while the affinity of the receptor slightly decreased. These results are the first to describe the number and affinity of PGE2 receptors on cells of the B lineage. These findings also suggest that B lymphocyte-activating molecules such as LPS and IL-4 may enhance sensitivity to PGE2 by upregulating the number of PGE2 receptors on B cells. Moreover, these observations may be of importance in developing strategies to specifically control the spread of PGE2-sensitive B lymphomas.

Original languageEnglish (US)
Pages (from-to)79-87
Number of pages9
JournalCellular Immunology
Volume161
Issue number1
DOIs
StatePublished - Mar 1995

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Prostaglandin Receptors
Dinoprostone
B-Lymphocytes
B-Cell Lymphoma
Cell Lineage
Interleukin-4
Lymphoma
Follicular Dendritic Cells
Immunoglobulin Class Switching
Cellular Microenvironment

ASJC Scopus subject areas

  • Immunology

Cite this

Characterization and regulation of prostaglandin E2 receptors on normal and malignant murine B lymphocytes. / Brown, Deborah M.; Phipps, Richard P.

In: Cellular Immunology, Vol. 161, No. 1, 03.1995, p. 79-87.

Research output: Contribution to journalArticle

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