Characterization and performance of a near-infrared 2-deoxyglucose optical imaging agent for mouse cancer models

Joy L. Kovar, William Volcheck, Eva Sevick-Muraca, Melanie A Simpson, D. Michael Olive

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Malignant neoplasms exhibit an elevated rate of glycolysis over normal cells. This characteristic can be exploited for optical imaging of tumors in mice. A near-infrared fluorophore, IRDye 800CW, emission maximum 794 nm, was conjugated to 2-deoxyglucose (2-DG). An immunofluorescent cell-based assay was used to evaluate specificity and sensitivity of the conjugate in cultured cell monolayers. Dose-dependent uptake was established with increasing concentrations of IRDye 800CW 2-DG for epithelial and prostate carcinomas. IRDye 800CW 2-DG was specifically blocked by an antibody against GLUT1 glucose transporter, and by excess unlabeled 2-DG or d-glucose. Signal was increased by a phorbol ester activator of glucose transport. Fluorescence microscopy data confirmed localization of the conjugate in the cytoplasm. Subsequent in vivo studies optimized dose, clearance, and timing for signal capture in nude mouse xenografts. In all cases, tumors were clearly imaged with good signal-to-noise characteristics. These data indicate that IRDye 800CW 2-DG is a broadly applicable optical imaging agent for in vivo imaging of neoplasms in mice.

Original languageEnglish (US)
Pages (from-to)254-262
Number of pages9
JournalAnalytical Biochemistry
Volume384
Issue number2
DOIs
StatePublished - Jan 15 2009

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Optical Imaging
Deoxyglucose
Infrared radiation
Imaging techniques
Neoplasms
Tumors
Glucose
Facilitative Glucose Transport Proteins
Fluorophores
Fluorescence microscopy
Phorbol Esters
Glycolysis
Fluorescence Microscopy
Heterografts
Nude Mice
Noise
Prostate
Cultured Cells
Monolayers
Assays

Keywords

  • 2-Deoxyglucose
  • Fluorescence microscopy
  • IRDye 800CW
  • Immunofluorescent assay
  • Molecular imaging
  • Near infrared
  • Whole animal imaging

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

Cite this

Characterization and performance of a near-infrared 2-deoxyglucose optical imaging agent for mouse cancer models. / Kovar, Joy L.; Volcheck, William; Sevick-Muraca, Eva; Simpson, Melanie A; Olive, D. Michael.

In: Analytical Biochemistry, Vol. 384, No. 2, 15.01.2009, p. 254-262.

Research output: Contribution to journalArticle

Kovar, Joy L. ; Volcheck, William ; Sevick-Muraca, Eva ; Simpson, Melanie A ; Olive, D. Michael. / Characterization and performance of a near-infrared 2-deoxyglucose optical imaging agent for mouse cancer models. In: Analytical Biochemistry. 2009 ; Vol. 384, No. 2. pp. 254-262.
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