Characterization and gene structure of a novel retinoblastoma-protein-associated protein similar to the transcription regulator TFII-I

Xiumin Yan, Xiangshan Zhao, Min Qian, Ning Guo, Xiaohua Gong, Xueliang Zhu

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Retinoblastoma protein (Rb) is an important regulator of vertebrate cell cycle and development. It functions through a direct interaction with protein factors involved in cell cycle progression and differentiation. In the present study we characterized a novel Rb-associated protein, Cream1, which bound to Rb specifically through a C-terminal region. Cream1 contained 959 amino acid residues and migrated as a protein of approx. 120 kDa on SDS/PAGE. It was a widely expressed nuclear protein with a nuclear localization signal resembling that of the large T antigen of simian virus 40. Its primary sequence was characteristic of five direct repeats that were similar to, but distinct from, those of TFII-I, a multifunctional transcription regulator. Three additional regions were also highly conserved in both proteins. Cream1 exhibited an activation activity that was attributed to its N-terminal portion when assayed in yeast. Its relationship with the muscle-enhancer-binding protein MusTRD1 further suggests a role in regulating gene expression. The structural gene, CREAM1, contained 27 exons and spanned more than 150 kb. It was located at human chromosome 7q11.23 in a region deleted for Williams' syndrome, a neurodevelopmental disease with multisystem abnormalities, implying its involvement in certain disorders. Taken together, our results suggest that Cream1 might serve as a positive transcription regulator under the control of Rb.

Original languageEnglish (US)
Pages (from-to)749-757
Number of pages9
JournalBiochemical Journal
Volume345
Issue number3
DOIs
StatePublished - Feb 1 2000

Fingerprint

Retinoblastoma Protein
Transcription
Genes
Cell Cycle
Williams Syndrome
Nuclear Localization Signals
Proteins
Simian virus 40
Nucleic Acid Repetitive Sequences
Viral Tumor Antigens
Cells
Human Chromosomes
Nuclear Proteins
Vertebrates
Polyacrylamide Gel Electrophoresis
Cell Differentiation
Exons
Carrier Proteins
Chromosomes
Yeasts

Keywords

  • MusTRD1
  • Nuclear protein
  • Williams' syndrome

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Characterization and gene structure of a novel retinoblastoma-protein-associated protein similar to the transcription regulator TFII-I. / Yan, Xiumin; Zhao, Xiangshan; Qian, Min; Guo, Ning; Gong, Xiaohua; Zhu, Xueliang.

In: Biochemical Journal, Vol. 345, No. 3, 01.02.2000, p. 749-757.

Research output: Contribution to journalArticle

Yan, Xiumin ; Zhao, Xiangshan ; Qian, Min ; Guo, Ning ; Gong, Xiaohua ; Zhu, Xueliang. / Characterization and gene structure of a novel retinoblastoma-protein-associated protein similar to the transcription regulator TFII-I. In: Biochemical Journal. 2000 ; Vol. 345, No. 3. pp. 749-757.
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