Characteristics of ribonucleic acids isolated from Botryodiplodia theobromae pycnidiospores

Robert H. Knight, James L Van Etten

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Nucleic acids isolated from dormant and germinated Botryodiplodia theobromae pycnidiospores contain five distinct species of RNA. They include two ribosomal species, two "ribosomal-associated" species and transfer RNAs. Sedimentation coefficients of 25.1S and 18S were obtained for the two ribosomal RNA species and 5.8S and 5S for the two "ribosomal-associated" RNA components. Molecular weights of 1.20, 0.67, 0.054 and 0.035x106 daltons were obtained after formaldehyde treatment and electrophoresis on polyacrylamide gels for these same four RNAs. Methylated nucleotides were present in the transfer RNAs and large and small ribosomal RNAs; in contrast 5.8S and 5S RNAs contained few methylated nucleotides. In addition to the 5 distinct RNA species, polyadenylate-containing RNA was isolated from both dormant and germinated spores.

Original languageEnglish (US)
Pages (from-to)45-50
Number of pages6
JournalArchives of Microbiology
Volume109
Issue number1-2
DOIs
StatePublished - Aug 1 1976

Fingerprint

RNA
Ribosomal RNA
Transfer RNA
5.8S Ribosomal RNA
5S Ribosomal RNA
Nucleotides
Spores
Nucleic Acids
Formaldehyde
Polyacrylamide Gel Electrophoresis
Electrophoresis
Sedimentation
Molecular Weight
Molecular weight

Keywords

  • Botryodiplodia theobromae
  • Fungal spores
  • RNA
  • Spore germination

ASJC Scopus subject areas

  • Microbiology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Characteristics of ribonucleic acids isolated from Botryodiplodia theobromae pycnidiospores. / Knight, Robert H.; Van Etten, James L.

In: Archives of Microbiology, Vol. 109, No. 1-2, 01.08.1976, p. 45-50.

Research output: Contribution to journalArticle

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N2 - Nucleic acids isolated from dormant and germinated Botryodiplodia theobromae pycnidiospores contain five distinct species of RNA. They include two ribosomal species, two "ribosomal-associated" species and transfer RNAs. Sedimentation coefficients of 25.1S and 18S were obtained for the two ribosomal RNA species and 5.8S and 5S for the two "ribosomal-associated" RNA components. Molecular weights of 1.20, 0.67, 0.054 and 0.035x106 daltons were obtained after formaldehyde treatment and electrophoresis on polyacrylamide gels for these same four RNAs. Methylated nucleotides were present in the transfer RNAs and large and small ribosomal RNAs; in contrast 5.8S and 5S RNAs contained few methylated nucleotides. In addition to the 5 distinct RNA species, polyadenylate-containing RNA was isolated from both dormant and germinated spores.

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KW - Spore germination

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