Characteristic differences in reverse transcription-polymerase chain reaction products of ovine, bovine, and human respiratory syncytial viruses

R. D. Oberst, M. P. Hays, J. F. Evermann, Clayton L Kelling

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10 Citations (Scopus)

Abstract

In reverse transcription-polymerase chain reactions (RT-PCR) and DNA hybridizations using primers and an oligonucleotide probe to the fusion (F) protein mRNA of bovine respiratory syncytial virus (BRSV), all the BRSV isolates and a goat isolate could be distinguished from prototype isolates of human respiratory syncytial viruses (HRSV) and ovine (sheep and bighorn sheep) respiratory syncytial viruses (RSV). However, RT-PCR amplifications with primers to sequences of the HRSV F protein mRNA resulted in amplified products of ≍ 243 bp if mRNA templates of subgroup A HRSV strains were present and slightly larger amplified products with subgroup B HRSV strains. No amplified products were observed in HRSV-primed RT-PCR with BRSV or goat or ovine RSV mRNA templates. Although the ovine RSV isolates were antigenically cross-reactive with the goat RSV, HRSV and BRSV isolates, they were not amplified with either HRSV- or BRSV-specific primers in RT-PCR. These results confirm previous immunological comparisons suggesting that some ovine RSV isolates should be considered as distinct respiratory syncytial viruses.

Original languageEnglish (US)
Pages (from-to)322-328
Number of pages7
JournalJournal of Veterinary Diagnostic Investigation
Volume5
Issue number3
DOIs
StatePublished - Jul 1993

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Pneumovirus
Bovine respiratory syncytial virus
Human respiratory syncytial virus
Respiratory Syncytial Viruses
Reverse Transcription
Sheep
reverse transcriptase polymerase chain reaction
Polymerase Chain Reaction
Goats
Messenger RNA
goats
Bighorn Sheep
Oligonucleotide Probes
sheep
Ovis canadensis
viruses
oligonucleotide probes
nucleic acid hybridization
prototypes
proteins

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Characteristic differences in reverse transcription-polymerase chain reaction products of ovine, bovine, and human respiratory syncytial viruses. / Oberst, R. D.; Hays, M. P.; Evermann, J. F.; Kelling, Clayton L.

In: Journal of Veterinary Diagnostic Investigation, Vol. 5, No. 3, 07.1993, p. 322-328.

Research output: Contribution to journalArticle

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