Changes in the structure and function of microbial communities in drinking water treatment bioreactors upon addition of phosphorus

Xu Li, Giridhar Upadhyaya, Wangki Yuen, Jess Brown, Eberhard Morgenroth, Lutgarde Raskin

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Phosphorus was added as a nutrient to bench-scale and pilot-scale biologically active carbon (BAC) reactors operated for perchlorate and nitrate removal from contaminated groundwater. The two bioreactors responded similarly to phosphorus addition in terms of microbial community function (i.e., reactor performance), while drastically different responses in microbial community structure were detected. Improvement in reactor performance with respect to perchlorate and nitrate removal started within a few days after phosphorus addition for both reactors. Microbial community structures were evaluated using molecular techniques targeting 16S rRNA genes. Clone library results showed that the relative abundance of perchlorate-reducing bacteria (PRB) Dechloromonas and Azospira in the bench-scale reactor increased from 15.2% and 0.6% to 54.2% and 11.7% after phosphorus addition, respectively. Real-time quantitative PCR (qPCR) experiments revealed that these increases started within a few days after phosphorus addition. In contrast, after phosphorus addition, the relative abundance of Dechloromonas in the pilot-scale reactor decreased from 7.1 to 0.6%, while Zoogloea increased from 17.9 to 52.0%. The results of this study demonstrated that similar operating conditions for bench-scale and pilot-scale reactors resulted in similar contaminant removal performances, despite dramatically different responses from microbial communities. These findings suggest that it is important toevaluate the microbial community compositions inside bioreactors used for drinking water treatment, as they determine the microbial composition in the effluent and impact downstream treatment requirements for drinking water production. This information could be particularly relevant to drinking water safety, if pathogens or disinfectant-resistant bacteria are detected in the bioreactors.

Original languageEnglish (US)
Pages (from-to)7473-7481
Number of pages9
JournalApplied and environmental microbiology
Volume76
Issue number22
DOIs
StatePublished - Nov 1 2010

Fingerprint

Water Purification
Bioreactors
bioreactors
water treatment
Drinking Water
bioreactor
Phosphorus
drinking water
microbial communities
microbial community
perchlorates
phosphorus
Dechloromonas
perchlorate
Nitrates
Azospira
Zoogloea
community structure
nitrates
relative abundance

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Changes in the structure and function of microbial communities in drinking water treatment bioreactors upon addition of phosphorus. / Li, Xu; Upadhyaya, Giridhar; Yuen, Wangki; Brown, Jess; Morgenroth, Eberhard; Raskin, Lutgarde.

In: Applied and environmental microbiology, Vol. 76, No. 22, 01.11.2010, p. 7473-7481.

Research output: Contribution to journalArticle

Li, Xu ; Upadhyaya, Giridhar ; Yuen, Wangki ; Brown, Jess ; Morgenroth, Eberhard ; Raskin, Lutgarde. / Changes in the structure and function of microbial communities in drinking water treatment bioreactors upon addition of phosphorus. In: Applied and environmental microbiology. 2010 ; Vol. 76, No. 22. pp. 7473-7481.
@article{f3ff1a73291d49ce92e148718f574607,
title = "Changes in the structure and function of microbial communities in drinking water treatment bioreactors upon addition of phosphorus",
abstract = "Phosphorus was added as a nutrient to bench-scale and pilot-scale biologically active carbon (BAC) reactors operated for perchlorate and nitrate removal from contaminated groundwater. The two bioreactors responded similarly to phosphorus addition in terms of microbial community function (i.e., reactor performance), while drastically different responses in microbial community structure were detected. Improvement in reactor performance with respect to perchlorate and nitrate removal started within a few days after phosphorus addition for both reactors. Microbial community structures were evaluated using molecular techniques targeting 16S rRNA genes. Clone library results showed that the relative abundance of perchlorate-reducing bacteria (PRB) Dechloromonas and Azospira in the bench-scale reactor increased from 15.2{\%} and 0.6{\%} to 54.2{\%} and 11.7{\%} after phosphorus addition, respectively. Real-time quantitative PCR (qPCR) experiments revealed that these increases started within a few days after phosphorus addition. In contrast, after phosphorus addition, the relative abundance of Dechloromonas in the pilot-scale reactor decreased from 7.1 to 0.6{\%}, while Zoogloea increased from 17.9 to 52.0{\%}. The results of this study demonstrated that similar operating conditions for bench-scale and pilot-scale reactors resulted in similar contaminant removal performances, despite dramatically different responses from microbial communities. These findings suggest that it is important toevaluate the microbial community compositions inside bioreactors used for drinking water treatment, as they determine the microbial composition in the effluent and impact downstream treatment requirements for drinking water production. This information could be particularly relevant to drinking water safety, if pathogens or disinfectant-resistant bacteria are detected in the bioreactors.",
author = "Xu Li and Giridhar Upadhyaya and Wangki Yuen and Jess Brown and Eberhard Morgenroth and Lutgarde Raskin",
year = "2010",
month = "11",
day = "1",
doi = "10.1128/AEM.01232-10",
language = "English (US)",
volume = "76",
pages = "7473--7481",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "22",

}

TY - JOUR

T1 - Changes in the structure and function of microbial communities in drinking water treatment bioreactors upon addition of phosphorus

AU - Li, Xu

AU - Upadhyaya, Giridhar

AU - Yuen, Wangki

AU - Brown, Jess

AU - Morgenroth, Eberhard

AU - Raskin, Lutgarde

PY - 2010/11/1

Y1 - 2010/11/1

N2 - Phosphorus was added as a nutrient to bench-scale and pilot-scale biologically active carbon (BAC) reactors operated for perchlorate and nitrate removal from contaminated groundwater. The two bioreactors responded similarly to phosphorus addition in terms of microbial community function (i.e., reactor performance), while drastically different responses in microbial community structure were detected. Improvement in reactor performance with respect to perchlorate and nitrate removal started within a few days after phosphorus addition for both reactors. Microbial community structures were evaluated using molecular techniques targeting 16S rRNA genes. Clone library results showed that the relative abundance of perchlorate-reducing bacteria (PRB) Dechloromonas and Azospira in the bench-scale reactor increased from 15.2% and 0.6% to 54.2% and 11.7% after phosphorus addition, respectively. Real-time quantitative PCR (qPCR) experiments revealed that these increases started within a few days after phosphorus addition. In contrast, after phosphorus addition, the relative abundance of Dechloromonas in the pilot-scale reactor decreased from 7.1 to 0.6%, while Zoogloea increased from 17.9 to 52.0%. The results of this study demonstrated that similar operating conditions for bench-scale and pilot-scale reactors resulted in similar contaminant removal performances, despite dramatically different responses from microbial communities. These findings suggest that it is important toevaluate the microbial community compositions inside bioreactors used for drinking water treatment, as they determine the microbial composition in the effluent and impact downstream treatment requirements for drinking water production. This information could be particularly relevant to drinking water safety, if pathogens or disinfectant-resistant bacteria are detected in the bioreactors.

AB - Phosphorus was added as a nutrient to bench-scale and pilot-scale biologically active carbon (BAC) reactors operated for perchlorate and nitrate removal from contaminated groundwater. The two bioreactors responded similarly to phosphorus addition in terms of microbial community function (i.e., reactor performance), while drastically different responses in microbial community structure were detected. Improvement in reactor performance with respect to perchlorate and nitrate removal started within a few days after phosphorus addition for both reactors. Microbial community structures were evaluated using molecular techniques targeting 16S rRNA genes. Clone library results showed that the relative abundance of perchlorate-reducing bacteria (PRB) Dechloromonas and Azospira in the bench-scale reactor increased from 15.2% and 0.6% to 54.2% and 11.7% after phosphorus addition, respectively. Real-time quantitative PCR (qPCR) experiments revealed that these increases started within a few days after phosphorus addition. In contrast, after phosphorus addition, the relative abundance of Dechloromonas in the pilot-scale reactor decreased from 7.1 to 0.6%, while Zoogloea increased from 17.9 to 52.0%. The results of this study demonstrated that similar operating conditions for bench-scale and pilot-scale reactors resulted in similar contaminant removal performances, despite dramatically different responses from microbial communities. These findings suggest that it is important toevaluate the microbial community compositions inside bioreactors used for drinking water treatment, as they determine the microbial composition in the effluent and impact downstream treatment requirements for drinking water production. This information could be particularly relevant to drinking water safety, if pathogens or disinfectant-resistant bacteria are detected in the bioreactors.

UR - http://www.scopus.com/inward/record.url?scp=78649717392&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78649717392&partnerID=8YFLogxK

U2 - 10.1128/AEM.01232-10

DO - 10.1128/AEM.01232-10

M3 - Article

VL - 76

SP - 7473

EP - 7481

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 22

ER -