Changes in soluble ribonucleic acid polymerases associated with the germination of Rhizopus stolonifer spores

Cheng Shung Gong, James L. Van Etten

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Germinated spores of Rhizopus stolonifer contained three soluble DNA-directed RNA polymerase enzymes (nucleoside triphosphate : RNA nucleotidyltransferase, EC 2.7.7.6) which were similar in many of their characteristics to those obtained from other eukaryotic organisms. Preparations from ungerminated spores yielded only RNA polymerases I and III. RNA polymerase III from both spore states exhibited identical characteristics, whereas RNA polymerase I from the ungerminated spores responded to divalent cations differently than the corresponding fraction from germinated spores. RNA polymerase II was first observed after the spores had undergone a swelling phase and they were ready to initiate germ tube formation. At the same time the characteristics of RNA polymerase I were altered so that they resembled those of the germinated spore enzyme.

Original languageEnglish (US)
Pages (from-to)44-52
Number of pages9
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume272
Issue number1
DOIs
StatePublished - Jun 22 1972

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Rhizopus
Germination
Spores
RNA
RNA Polymerase I
RNA Polymerase III
RNA Nucleotidyltransferases
RNA Polymerase II
Divalent Cations
Enzymes
DNA-Directed RNA Polymerases
Nucleosides

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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abstract = "Germinated spores of Rhizopus stolonifer contained three soluble DNA-directed RNA polymerase enzymes (nucleoside triphosphate : RNA nucleotidyltransferase, EC 2.7.7.6) which were similar in many of their characteristics to those obtained from other eukaryotic organisms. Preparations from ungerminated spores yielded only RNA polymerases I and III. RNA polymerase III from both spore states exhibited identical characteristics, whereas RNA polymerase I from the ungerminated spores responded to divalent cations differently than the corresponding fraction from germinated spores. RNA polymerase II was first observed after the spores had undergone a swelling phase and they were ready to initiate germ tube formation. At the same time the characteristics of RNA polymerase I were altered so that they resembled those of the germinated spore enzyme.",
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