Changes in protein abundance during powdery mildew infection of leaf tissues of Cabernet Sauvignon grapevine (Vitis vinifera L.)

Ellen Marsh, Sophie Alvarez, Leslie M. Hicks, W. Brad Barbazuk, Wenping Qiu, Laszlo Kovacs, Daniel Schachtman

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

A comparative analysis of differentially expressed proteins in a susceptible grapevine (Vitis vinifera 'Cabernet Sauvignon') during the infection of Erysiphe necator, the causal pathogen of grapevine powdery mildew (PM), was conducted using iTRAQ. The quantitative labeling analysis revealed 63 proteins that significantly changed in abundance at 24, 36, 48, and 72 h post inoculation with powdery mildew conidiospores. The functional classification of the PMresponsive proteins showed that they are involved in photosynthesis, metabolism, disease/defense, protein destination, and protein synthesis. A number of the proteins induced in grapevine in response to E. necator are associated with the plant defense response, suggesting that PM-susceptible Cabernet Sauvignon is able to initiate a basal defense but unable to restrict fungal growth or slow down disease progression.

Original languageEnglish (US)
Pages (from-to)2057-2064
Number of pages8
JournalProteomics
Volume10
Issue number10
DOIs
StatePublished - May 1 2010

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Vitis
Tissue
Necator
Infection
Proteins
Photosynthesis
Pathogens
Metabolism
Labeling
Disease Progression
Growth

Keywords

  • Biotic stress
  • Plant proteomics
  • Powdery mildew
  • Vitis vinifera
  • iTRAQ

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Changes in protein abundance during powdery mildew infection of leaf tissues of Cabernet Sauvignon grapevine (Vitis vinifera L.). / Marsh, Ellen; Alvarez, Sophie; Hicks, Leslie M.; Barbazuk, W. Brad; Qiu, Wenping; Kovacs, Laszlo; Schachtman, Daniel.

In: Proteomics, Vol. 10, No. 10, 01.05.2010, p. 2057-2064.

Research output: Contribution to journalArticle

Marsh, Ellen ; Alvarez, Sophie ; Hicks, Leslie M. ; Barbazuk, W. Brad ; Qiu, Wenping ; Kovacs, Laszlo ; Schachtman, Daniel. / Changes in protein abundance during powdery mildew infection of leaf tissues of Cabernet Sauvignon grapevine (Vitis vinifera L.). In: Proteomics. 2010 ; Vol. 10, No. 10. pp. 2057-2064.
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