Changes in fluorescence spectra of bioaerosols exposed to ozone in a laboratory reaction chamber to simulate atmospheric aging

Joshua L. Santarpia, Yong Le Pan, Steven C. Hill, Neal Baker, Brian Cottrell, Laura McKee, Shanna Ratnesar-Shumate, Ronald G. Pinnick

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A laboratory system for exposing aerosol particles to ozone and rapidly measuring the subsequent changes in their single-particle fluorescence is reported. The system consists of a rotating drum chamber and a single-particle fluorescence spectrometer (SPFS) utilizing excitation at 263 nm. Measurements made with this system show preliminary results on the ultra-violet laser-induced-fluorescence (UV-LIF) spectra of single aerosolized particles of Yersinia rohdei, and of MS2 (bacteriophage) exposed to ozone. When bioparticles are exposed in the chamber the fluorescence emission peak around 330 nm: i) decreases in intensity relative to that of the 400-550 nm band; and ii) shifts slightly toward shorterwavelengths (consistent with further drying of the particles). In these experiments, changes were observed at exposures below the US Environmental Protection Agency (EPA) limits for ozone

Original languageEnglish (US)
Pages (from-to)29867-29881
Number of pages15
JournalOptics Express
Volume20
Issue number28
DOIs
StatePublished - Dec 31 2012

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ozone
chambers
fluorescence
bacteriophages
drums
ultraviolet lasers
laser induced fluorescence
drying
aerosols
spectrometers
shift
excitation

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Changes in fluorescence spectra of bioaerosols exposed to ozone in a laboratory reaction chamber to simulate atmospheric aging. / Santarpia, Joshua L.; Pan, Yong Le; Hill, Steven C.; Baker, Neal; Cottrell, Brian; McKee, Laura; Ratnesar-Shumate, Shanna; Pinnick, Ronald G.

In: Optics Express, Vol. 20, No. 28, 31.12.2012, p. 29867-29881.

Research output: Contribution to journalArticle

Santarpia, JL, Pan, YL, Hill, SC, Baker, N, Cottrell, B, McKee, L, Ratnesar-Shumate, S & Pinnick, RG 2012, 'Changes in fluorescence spectra of bioaerosols exposed to ozone in a laboratory reaction chamber to simulate atmospheric aging', Optics Express, vol. 20, no. 28, pp. 29867-29881. https://doi.org/10.1364/OE.20.029867
Santarpia, Joshua L. ; Pan, Yong Le ; Hill, Steven C. ; Baker, Neal ; Cottrell, Brian ; McKee, Laura ; Ratnesar-Shumate, Shanna ; Pinnick, Ronald G. / Changes in fluorescence spectra of bioaerosols exposed to ozone in a laboratory reaction chamber to simulate atmospheric aging. In: Optics Express. 2012 ; Vol. 20, No. 28. pp. 29867-29881.
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