Centrally mediated erectile dysfunction in rats with type 1 diabetes

Role of angiotensin II and superoxide

Hong Zheng, Xuefei Liu, Kaushik P Patel

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Introduction: Erectile dysfunction is a serious complication of diabetes mellitus. Apart from the peripheral actions, central mechanisms are also responsible for penile erection. Aim: This study aims to determine the contribution of angiotensin (ANG) II in the dysfunction of central N-methyl-D-aspartic acid (NMDA)- and nitric oxide (NO)-induced erectile responses in streptozotocin-induced type 1 diabetic (T1D) rats. Methods: Three weeks after streptozotocin injections, rats were randomly treated with the angiotensin-converting enzyme inhibitor-enalapril, or the ANG II type 1 receptor blocker, losartan, or the superoxide dismutase mimetic, tempol, or vehicle via chronic intracerebroventricular infusion by osmotic mini-pump for 2 weeks. Main Outcome Measure: Central NMDA receptor stimulation or the administration of the NO donor, sodium nitroprusside (SNP)-induced penile erectile responses and concurrent behavioral responses were monitored in conscious rats. Results: Two weeks of enalapril, losartan, or tempol treatment significantly improved the erectile responses to central microinjection of both NMDA and SNP in the paraventricular nucleus (PVN) of conscious T1D rats (NMDA responses-T1D+enalapril: 1.7±0.6, T1D+losartan: 2.0±0.3, T1D+tempol: 2.0±0.6 vs. T1D+vehicle: 0.6±0.3 penile erections/rat in the first 20 minutes, P<0.05; SNP responses-T1D+enalapril: 0.9±0.3, T1D+losartan: 1.3±0.3, T1D+tempol: 1.4±0.4 vs. T1D+vehicle: 0.4±0.2 penile erections/rat in the first 20 minutes, P<0.05). Concurrent behavioral responses including yawning and stretching, induced by central NMDA and SNP microinjections, were also significantly increased in T1D rats after enalapril, losartan, or tempol treatments. Neuronal NO synthase expression within the PVN was also significantly increased, and superoxide production was reduced in T1D rats after these treatments. Conclusions: These data strongly support the contention that enhanced ANG II mechanism/s within the PVN of T1D rats contributes to the dysfunction of central NMDA-induced erectile responses in T1D rats via stimulation of superoxide. Zheng H, Liu X, and Patel KP. Centrally mediated erectile dysfunction in rats with type 1 diabetes: Role of angiotensin II and superoxide. J Sex Med 2013;10:2165-2176.

Original languageEnglish (US)
Pages (from-to)2165-2176
Number of pages12
JournalJournal of Sexual Medicine
Volume10
Issue number9
DOIs
StatePublished - Jan 1 2013

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Erectile Dysfunction
Type 1 Diabetes Mellitus
Superoxides
Angiotensin II
N-Methylaspartate
Enalapril
Losartan
Nitroprusside
Penile Erection
Paraventricular Hypothalamic Nucleus
Microinjections
Streptozocin
Yawning
Intraventricular Infusions
Angiotensin II Type 1 Receptor Blockers
Nitric Oxide Synthase Type I
Nitric Oxide Donors
Diabetes Complications
Angiotensin-Converting Enzyme Inhibitors
Superoxide Dismutase

Keywords

  • Central Mechanisms of Penile Erection
  • Central Nervous System
  • Erectile Dysfunction
  • Type 1 Diabetes

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology
  • Urology

Cite this

Centrally mediated erectile dysfunction in rats with type 1 diabetes : Role of angiotensin II and superoxide. / Zheng, Hong; Liu, Xuefei; Patel, Kaushik P.

In: Journal of Sexual Medicine, Vol. 10, No. 9, 01.01.2013, p. 2165-2176.

Research output: Contribution to journalArticle

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