Central sprouting and functional plasticity of regenerated primary afferents

H. R. Koerber, Karoly Mirnics, P. B. Brown, L. M. Mendell

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

A combination of neuroanatomical and electrophysiological techniques was used to study the effects of peripheral axotomy and regeneration of primary afferents on their central projections in the spinal cord. Individual regenerated afferent fibers were impaled with HRP-filled electrodes in the dorsal columns of α-chloralose-anesthetized cats and activated by current pulses delivered via the intracellular electrode. The resulting cord dorsum potentials (CDPs) were recorded at four rostrocaudal locations and HRP was iontophoretically injected into the fiber. Central distributions of boutons and CDPs were compared with peripheral receptor type to determine the accuracy of peripheral regeneration and the effects of central-peripheral mismatches. Reconstruction of the central projections of 13 individual afferents for which the adequate stimulus and CDPs had been recorded revealed many abnormalities. For example, unlike controls, four group I and II afferents with central projections typical of proprioceptors (concentrated in laminae V, VI, and VII) innervating either cutaneous or noncutaneous targets evoked measurable CDPs. Three other group II or Aβ afferents innervating low-threshold mechanoreceptors with central terminations confined to the dorsal horn exhibited extensive collateralization in laminae I and II in addition to large numbers of terminals in laminae III-IV. These fibers activated central networks whose adaptation behavior was identical to those evoked by high-threshold mechanoreceptive afferents in controls. These results suggest that primary afferents and their central connections are capable of significant modifications following axotomy and regeneration. In addition, the anatomical studies indicate some reorganization in the laminar distribution of boutons as well as in bouton size.

Original languageEnglish (US)
Pages (from-to)3655-3671
Number of pages17
JournalJournal of Neuroscience
Volume14
Issue number6
StatePublished - Jun 1 1994

Fingerprint

Axotomy
Regeneration
Electrodes
Substantia Gelatinosa
Chloralose
Mechanoreceptors
Spinal Cord
Cats
Control Groups
Skin
Spinal Cord Dorsal Horn

Keywords

  • axotomy
  • plasticity
  • primary afferents
  • regeneration
  • spinal cord
  • sprouting

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Koerber, H. R., Mirnics, K., Brown, P. B., & Mendell, L. M. (1994). Central sprouting and functional plasticity of regenerated primary afferents. Journal of Neuroscience, 14(6), 3655-3671.

Central sprouting and functional plasticity of regenerated primary afferents. / Koerber, H. R.; Mirnics, Karoly; Brown, P. B.; Mendell, L. M.

In: Journal of Neuroscience, Vol. 14, No. 6, 01.06.1994, p. 3655-3671.

Research output: Contribution to journalArticle

Koerber, HR, Mirnics, K, Brown, PB & Mendell, LM 1994, 'Central sprouting and functional plasticity of regenerated primary afferents', Journal of Neuroscience, vol. 14, no. 6, pp. 3655-3671.
Koerber, H. R. ; Mirnics, Karoly ; Brown, P. B. ; Mendell, L. M. / Central sprouting and functional plasticity of regenerated primary afferents. In: Journal of Neuroscience. 1994 ; Vol. 14, No. 6. pp. 3655-3671.
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