Cellular transcription factors induced in trigeminal ganglia during dexamethasone-induced reactivation from latency stimulate bovine herpesvirus 1 productive infection and certain viral promoters

Aspen Workman, James D Eudy, Lynette M Smith, Leticia Frizzo da Silva, Devis Sinani, Halie Bricker, Emily Cook, Alan R Doster, Clinton Jones

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Bovine herpesvirus 1 (BHV-1), an alphaherpesvirinae subfamily member, establishes latency in sensory neurons. Elevated corticosteroid levels, due to stress, reproducibly triggers reactivation from latency in the field. A single intravenous injection of the synthetic corticosteroid dexamethasone (DEX) to latently infected calves consistently induces reactivation from latency. Lytic cycle viral gene expression is detected in sensory neurons within 6 h after DEX treatment of latently infected calves. These observations suggested that DEX stimulated expression of cellular genes leads to lytic cycle viral gene expression and productive infection. In this study, a commercially available assay-Bovine Gene Chip-was used to compare cellular gene expression in the trigeminal ganglia (TG) of calves latently infected with BHV-1 versus DEX-treated animals. Relative to TG prepared from latently infected calves, 11 cellular genes were induced more than 10-fold 3 h after DEX treatment. Pentraxin three, a regulator of innate immunity and neurodegeneration, was stimulated 35- to 63-fold after 3 or 6 h of DEXtreatment. Two transcription factors, promyelocytic leukemia zinc finger (PLZF) and Slug were induced more than 15-fold 3 h after DEX treatment. PLZF or Slug stimulated productive infection 20- or 5-fold, respectively, and Slug stimulated the late glycoprotein C promoter more than 10-fold. Additional DEX-induced transcription factors also stimulated productive infection and certain viral promoters. These studies suggest that DEX-inducible cellular transcription factors and/or signaling pathways stimulate lytic cycle viral gene expression, which subsequently leads to successful reactivation from latency in a small subset of latently infected neurons.

Original languageEnglish (US)
Pages (from-to)2459-2473
Number of pages15
JournalJournal of virology
Volume86
Issue number5
DOIs
StatePublished - Mar 1 2012

Fingerprint

Bovine Herpesvirus 1
Trigeminal Ganglion
Bovine herpesvirus 1
Virus Diseases
dexamethasone
Dexamethasone
Transcription Factors
transcription factors
promoter regions
infection
Gastropoda
Viral Genes
slugs
Gene Expression
calves
gene expression
sensory neurons
zinc finger motif
Zinc Fingers
Sensory Receptor Cells

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Cellular transcription factors induced in trigeminal ganglia during dexamethasone-induced reactivation from latency stimulate bovine herpesvirus 1 productive infection and certain viral promoters. / Workman, Aspen; Eudy, James D; Smith, Lynette M; da Silva, Leticia Frizzo; Sinani, Devis; Bricker, Halie; Cook, Emily; Doster, Alan R; Jones, Clinton.

In: Journal of virology, Vol. 86, No. 5, 01.03.2012, p. 2459-2473.

Research output: Contribution to journalArticle

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