Cellular level robotic surgery

Nanodissection of intermediate filaments in live keratinocytes

Ruiguo Yang, Bo Song, Zhiyong Sun, King Wai Chiu Lai, Carmen Kar Man Fung, Kevin C. Patterson, Kristina Seiffert-Sinha, Animesh A. Sinha, Ning Xi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We present the nanosurgery on the cytoskeleton of live cells using AFM based nanorobotics to achieve adhesiolysis and mimic the effect of pathophysiological modulation of intercellular adhesion. Nanosurgery successfully severs the intermediate filament bundles and disrupts cell-cell adhesion similar to the desmosomal protein disassembly in autoimmune disease, or the cationic modulation of desmosome formation. Our nanomechanical analysis revealed that adhesion loss results in a decrease in cellular stiffness in both cases of biochemical modulation of the desmosome junctions and mechanical disruption of intercellular adhesion, supporting the notion that intercellular adhesion through intermediate filaments anchors the cell structure as focal adhesion does and that intermediate filaments are integral components in cell mechanical integrity. The surgical process could potentially help reveal the mechanism of autoimmune pathology-induced cell-cell adhesion loss as well as its related pathways that lead to cell apoptosis. From the Clinical Editor: This team of authors performed nanosurgery on the cytoskeleton of live cells using AFM based nanorobotics to achieve adhesiolysis, and mimic the effect of pathophysiological modulation of intercellular adhesions. This method could potentially help reveal the mechanism of autoimmune pathology-induced cell-cell adhesion loss as well as its related pathways that lead to cell apoptosis.

Original languageEnglish (US)
Pages (from-to)137-145
Number of pages9
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume11
Issue number1
DOIs
StatePublished - Jan 1 2015

Fingerprint

Intermediate Filaments
Robotics
Keratinocytes
Adhesion
Cell adhesion
Nanorobotics
Modulation
Cell Adhesion
Pathology
Cell death
Desmosomes
Cytoskeleton
Apoptosis
Anchors
Focal Adhesions
Robotic surgery
Cellular Structures
Stiffness
Autoimmune Diseases
Proteins

Keywords

  • Atomic Force Microscopy
  • Cell-cell adhesion
  • Desmosome
  • Intermediate filament
  • Mechanical property
  • Nanosurgery

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

Cite this

Cellular level robotic surgery : Nanodissection of intermediate filaments in live keratinocytes. / Yang, Ruiguo; Song, Bo; Sun, Zhiyong; Lai, King Wai Chiu; Fung, Carmen Kar Man; Patterson, Kevin C.; Seiffert-Sinha, Kristina; Sinha, Animesh A.; Xi, Ning.

In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 11, No. 1, 01.01.2015, p. 137-145.

Research output: Contribution to journalArticle

Yang, R, Song, B, Sun, Z, Lai, KWC, Fung, CKM, Patterson, KC, Seiffert-Sinha, K, Sinha, AA & Xi, N 2015, 'Cellular level robotic surgery: Nanodissection of intermediate filaments in live keratinocytes', Nanomedicine: Nanotechnology, Biology, and Medicine, vol. 11, no. 1, pp. 137-145. https://doi.org/10.1016/j.nano.2014.08.008
Yang, Ruiguo ; Song, Bo ; Sun, Zhiyong ; Lai, King Wai Chiu ; Fung, Carmen Kar Man ; Patterson, Kevin C. ; Seiffert-Sinha, Kristina ; Sinha, Animesh A. ; Xi, Ning. / Cellular level robotic surgery : Nanodissection of intermediate filaments in live keratinocytes. In: Nanomedicine: Nanotechnology, Biology, and Medicine. 2015 ; Vol. 11, No. 1. pp. 137-145.
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