Cellular distribution of lens epithelium-derived growth factor (LEDGF) in the rat eye: Loss of LEDGF from nuclei of differentiating cells

Eri Kubo, Dhirendra P. Singh, Nigar Fatma, Toshimichi Shinohara, Peggy Zelenka, Venkat N. Reddy, Leo T. Chylack

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Lens epithelium-derived growth factor (LEDGF) enhances the survival and growth of cells. To understand LEDGF's spatial localization and its putative function(s) during proliferation and differentiation, we localized LEDGF during terminal differentiation in whole rat lenses, lens epithelial cell (LEC) explants stimulated with FGF-2, and insulin, iris, human LECs with lentoids. In addition, intracellular localization of LEDGF was performed in other ocular tissues: ciliary body, retina, and cornea. We found the immunopositivity of nuclear LEDGF decreased in LECs of the equatorial region. In contrast, immunopositivity of LEDGF was detected in the cytoplasm of LECs and superficial fiber cells. After treating LEC explants with FGF-2 and insulin, which are known to be differentiating factors for LECs, the nuclei of these cells showed no LEDGF immunopositivity, but explants did express p57kip2, a differentiation marker protein. Also, immunopositive LEDGF was not detected in the nuclei of differentiated cells, lentoid body, and corneal epithelial cells. This demonstrated that the loss of LEDGF from the nucleus may be associated with the process of terminal differentiation that might be in some way common with the biochemical mechanisms of apoptosis. The spatial and temporal distribution of LEDGF in the present study also provides a vision for further investigation as to how this protein is involved in cell fate determination.

Original languageEnglish (US)
Pages (from-to)289-299
Number of pages11
JournalHistochemistry and Cell Biology
Volume119
Issue number4
StatePublished - Apr 1 2003

Fingerprint

Crystalline Lens
epithelium
Cell Nucleus
rats
Rats
Lenses
lenses
nuclei
cells
Epithelial Cells
Fibroblast Growth Factor 2
insulin
Insulin
lens epithelium-derived growth factor
Intercellular Signaling Peptides and Proteins
Ciliary Body
proteins
Proteins
Differentiation Antigens
Iris

Keywords

  • Differentiation
  • Immunohistochemistry
  • In situ hybridization
  • LEC explants
  • LEDGF
  • Lentoid body

ASJC Scopus subject areas

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

Cite this

Cellular distribution of lens epithelium-derived growth factor (LEDGF) in the rat eye : Loss of LEDGF from nuclei of differentiating cells. / Kubo, Eri; Singh, Dhirendra P.; Fatma, Nigar; Shinohara, Toshimichi; Zelenka, Peggy; Reddy, Venkat N.; Chylack, Leo T.

In: Histochemistry and Cell Biology, Vol. 119, No. 4, 01.04.2003, p. 289-299.

Research output: Contribution to journalArticle

Kubo, Eri ; Singh, Dhirendra P. ; Fatma, Nigar ; Shinohara, Toshimichi ; Zelenka, Peggy ; Reddy, Venkat N. ; Chylack, Leo T. / Cellular distribution of lens epithelium-derived growth factor (LEDGF) in the rat eye : Loss of LEDGF from nuclei of differentiating cells. In: Histochemistry and Cell Biology. 2003 ; Vol. 119, No. 4. pp. 289-299.
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