Cellular distribution of HLA-G mRNA in transgenic mouse placentas

K. K. Yelavarthi, C. M. Schmidt, R. G. Ehlenfeldt, H. T. Orr, J. S. Hunt

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The human MHC class I gene, HLA-G, is unique among members of the class I gene family in that it is nonpolymorphic, and expression is primarily restricted to extraembryonic tissues. To examine regulatory elements that direct tissue- and cell lineage-specific expression of this gene, transgenic mice expressing HLA-G have been established. In this study, in situ hybridization was used to evaluate the cellular distribution of HLA-G mRNA in transgenic placentas. Extraembryonic tissues were obtained at gestation day 12.5 from embryos that had been microinjected with either 6.0 or 5.7 kb of HLA-G genomic DNA and had been transferred into pseudopregnant HLA-G transgenic mice or Swiss mice. The 6.0 kb transgene contained an additional 250 bp at the extreme 5'-end of the upstream region. Genotype of the recipient had no discernable effect on the cellular distribution of HLA-G mRNA. HLA-G mRNA was present in both trophoblast and mesenchymal cells in transgenic placentas carrying 6.0 kb of genomic HLA-G, a pattern strikingly similar to that of HLA-G message distribution in early gestation human placentas. By contrast, in placentas from embryos carrying 5.7 kb HLA-G DNA, specific mRNA was found primarily in mesenchymal cells at the base of the placenta. Thus, the 6.0 genomic fragment contains elements capable of directing HLA-G expression in placentas, and is particularly influential in the trophoblastic cell lineage.

Original languageEnglish (US)
Pages (from-to)3638-3645
Number of pages8
JournalJournal of Immunology
Volume151
Issue number7
StatePublished - Jan 1 1993

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HLA-G Antigens
Placenta
Transgenic Mice
Messenger RNA
MHC Class I Genes
Cell Lineage
Embryonic Structures
Pregnancy
DNA
Trophoblasts
Transgenes
In Situ Hybridization

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Yelavarthi, K. K., Schmidt, C. M., Ehlenfeldt, R. G., Orr, H. T., & Hunt, J. S. (1993). Cellular distribution of HLA-G mRNA in transgenic mouse placentas. Journal of Immunology, 151(7), 3638-3645.

Cellular distribution of HLA-G mRNA in transgenic mouse placentas. / Yelavarthi, K. K.; Schmidt, C. M.; Ehlenfeldt, R. G.; Orr, H. T.; Hunt, J. S.

In: Journal of Immunology, Vol. 151, No. 7, 01.01.1993, p. 3638-3645.

Research output: Contribution to journalArticle

Yelavarthi, KK, Schmidt, CM, Ehlenfeldt, RG, Orr, HT & Hunt, JS 1993, 'Cellular distribution of HLA-G mRNA in transgenic mouse placentas', Journal of Immunology, vol. 151, no. 7, pp. 3638-3645.
Yelavarthi KK, Schmidt CM, Ehlenfeldt RG, Orr HT, Hunt JS. Cellular distribution of HLA-G mRNA in transgenic mouse placentas. Journal of Immunology. 1993 Jan 1;151(7):3638-3645.
Yelavarthi, K. K. ; Schmidt, C. M. ; Ehlenfeldt, R. G. ; Orr, H. T. ; Hunt, J. S. / Cellular distribution of HLA-G mRNA in transgenic mouse placentas. In: Journal of Immunology. 1993 ; Vol. 151, No. 7. pp. 3638-3645.
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