Cellular biophysical dynamics and ion channel activities detected by AFM-based nanorobotic manipulator in insulinoma β-cells

Ruiguo Yang, Ning Xi, King Wai Chiu Lai, Kevin C. Patterson, Hongzhi Chen, Bo Song, Chengeng Qu, Beihua Zhong, Donna H. Wang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Distinct biochemical, electrochemical and electromechanical coupling processes of pancreatic β-cells may well underlie different response patterns of insulin release from glucose and capsaicin stimulation. Intracellular Ca2+ levels increased rapidly and dose-dependently upon glucose stimulation, accompanied with about threefold rapid increases in cellular stiffness. Subsequently, cellular stiffness diminished rapidly and settled at a value about twofold of the baseline. Capsaicin caused a similar transient increase in intracellular Ca2+ changes. However, cellular stiffness increased gradually to about twofold until leveling off. The current study characterizes for the first time the biophysical properties underlying glucose-induced biphasic responses of insulin secretion, distinctive from the slow and single-phased stiffness response to capsaicin despite similar changes in intracellular Ca2+ levels. The integrated AFM nanorobotics and optical investigation enables the fine dissection of mechano-property from ion channel activities in response to specific and non-specific agonist stimulation, providing novel biomechanical markers for the insulin secretion process. From the Clinical Editor: This study characterizes the biophysical properties underlying glucose-induced biphasic responses of insulin secretion. Integrated AFM nanorobotics and optical investigations provided novel biomechanical markers for the insulin secretion process.

Original languageEnglish (US)
Pages (from-to)636-645
Number of pages10
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume9
Issue number5
DOIs
StatePublished - Jul 1 2013

Fingerprint

Nanorobotics
Insulinoma
Insulin
Ion Channels
Manipulators
Capsaicin
Biphasic Insulins
Glucose
Stiffness
Secretory Pathway
Ions
Dissection
Electromechanical coupling

Keywords

  • AFM nanorobotics
  • Cellular stiffness
  • Insulinoma β-cells
  • Ion channel activities

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

Cite this

Cellular biophysical dynamics and ion channel activities detected by AFM-based nanorobotic manipulator in insulinoma β-cells. / Yang, Ruiguo; Xi, Ning; Lai, King Wai Chiu; Patterson, Kevin C.; Chen, Hongzhi; Song, Bo; Qu, Chengeng; Zhong, Beihua; Wang, Donna H.

In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 9, No. 5, 01.07.2013, p. 636-645.

Research output: Contribution to journalArticle

Yang, Ruiguo ; Xi, Ning ; Lai, King Wai Chiu ; Patterson, Kevin C. ; Chen, Hongzhi ; Song, Bo ; Qu, Chengeng ; Zhong, Beihua ; Wang, Donna H. / Cellular biophysical dynamics and ion channel activities detected by AFM-based nanorobotic manipulator in insulinoma β-cells. In: Nanomedicine: Nanotechnology, Biology, and Medicine. 2013 ; Vol. 9, No. 5. pp. 636-645.
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