Cell wall proteome in the maize primary root elongation zone. II. Region-specific changes in water soluble and lightly ionically bound proteins under water deficit

Jinming Zhu, Sophie Alvarez, Ellen L. Marsh, Mary E. LeNoble, In Jeong Cho, Mayandi Sivaguru, Sixue Chen, Henry T. Nguyen, Yajun Wu, Daniel P. Schachtman, Robert E. Sharp

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

Previous work on the adaptation of maize (Zea mays) primary roots to water deficit showed that cell elongation is maintained preferentially toward the apex, and that this response involves modification of cell wall extension properties. To gain a comprehensive understanding of how cell wall protein (CWP) composition changes in association with the differential growth responses to water deficit in different regions of the elongation zone, a proteomics approach was used to examine water soluble and loosely ionically bound CWPs. The results revealed major and predominantly region-specific changes in protein profiles between well-watered and water-stressed roots. In total, 152 water deficit-responsive proteins were identified and categorized into five groups based on their potential function in the cell wall: reactive oxygen species (ROS) metabolism, defense and detoxification, hydrolases, carbohydrate metabolism, and other/unknown. The results indicate that stress-induced changes in CWPs involve multiple processes that are likely to regulate the response of cell elongation. In particular, the changes in protein abundance related to ROS metabolism predicted an increase in apoplastic ROS production in the apical region of the elongation zone of water-stressed roots. This was verified by quantification of hydrogen peroxide content in extracted apoplastic fluid and by in situ imaging of apoplastic ROS levels. This response could contribute directly to the enhancement of wall loosening in this region. This large-scale proteomic analysis provides novel insights into the complexity of mechanisms that regulate root growth under water deficit conditions and highlights the spatial differences in CWP composition in the root elongation zone.

Original languageEnglish (US)
Pages (from-to)1533-1548
Number of pages16
JournalPlant physiology
Volume145
Issue number4
DOIs
StatePublished - Dec 1 2007

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Proteome
proteome
Cell Wall
Zea mays
root growth
cell walls
corn
Water
reactive oxygen species
Reactive Oxygen Species
Proteins
proteins
water
protein composition
Proteomics
proteomics
cell growth
metabolism
Carbohydrate Metabolism
Hydrolases

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Cell wall proteome in the maize primary root elongation zone. II. Region-specific changes in water soluble and lightly ionically bound proteins under water deficit. / Zhu, Jinming; Alvarez, Sophie; Marsh, Ellen L.; LeNoble, Mary E.; Cho, In Jeong; Sivaguru, Mayandi; Chen, Sixue; Nguyen, Henry T.; Wu, Yajun; Schachtman, Daniel P.; Sharp, Robert E.

In: Plant physiology, Vol. 145, No. 4, 01.12.2007, p. 1533-1548.

Research output: Contribution to journalArticle

Zhu, J, Alvarez, S, Marsh, EL, LeNoble, ME, Cho, IJ, Sivaguru, M, Chen, S, Nguyen, HT, Wu, Y, Schachtman, DP & Sharp, RE 2007, 'Cell wall proteome in the maize primary root elongation zone. II. Region-specific changes in water soluble and lightly ionically bound proteins under water deficit', Plant physiology, vol. 145, no. 4, pp. 1533-1548. https://doi.org/10.1104/pp.107.107250
Zhu, Jinming ; Alvarez, Sophie ; Marsh, Ellen L. ; LeNoble, Mary E. ; Cho, In Jeong ; Sivaguru, Mayandi ; Chen, Sixue ; Nguyen, Henry T. ; Wu, Yajun ; Schachtman, Daniel P. ; Sharp, Robert E. / Cell wall proteome in the maize primary root elongation zone. II. Region-specific changes in water soluble and lightly ionically bound proteins under water deficit. In: Plant physiology. 2007 ; Vol. 145, No. 4. pp. 1533-1548.
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