Cell fractionation of large bowel cancer

Michael G. Brattain, Theresa P. Pretlow, Thomas G. Pretlow

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The fractionation of cells from human colonic carcinoma, a transplantable colonic carcinoma of the rat, and normal rat colonic mucosa by centrifugation in linear gradients of Ficoll (polysucrose) in tissue culture medium is described. Epithelial cells from human colonic carcinoma showed strong histochemical N‐acety‐β‐D‐glucosaminidase activity relative to stromal cells. The histochemical demonstration of this enzyme was employed to monitor the separation of malignant cells from other types of cells in human colonic carcinomas. After centrifugation, the cells from the quarter of the density gradient containing the highest percentage of cells with histochemically demonstrable N‐acetyl‐β‐D‐glucosaminidase also contained the highest levels of CEA/cell. Cells from this zone of the gradient gave rise to colonies when cultured in soft agar. Suspensions of cells from human colonic carcinomas contained eosinophils, plasma cells, lymphocytes, red blood cells and several unidentified morphological variants, which we believe to be of an epithelial nature. After centrifugation these morphological variants were located in the zone of the gradient that gave rise to colonies when cultured in soft agar. Similar morphological varieties were observed in both normal rat colonic mucosa and the transplantable rat tumor. The advantages of separated cells for a) the preparation of colonic cells for tissue culture and b) biochemical characterizations of malignant cells are described.

Original languageEnglish (US)
Pages (from-to)2479-2486
Number of pages8
JournalCancer
Volume40
Issue number5 S
DOIs
StatePublished - Nov 1977

Fingerprint

Cell Fractionation
Colonic Neoplasms
Carcinoma
Centrifugation
Agar
Mucous Membrane
Ficoll
Cell Separation
Stromal Cells
Plasma Cells
Eosinophils
Culture Media
Suspensions
Cell Culture Techniques
Cell Count
Erythrocytes
Epithelial Cells
Lymphocytes
Enzymes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Cell fractionation of large bowel cancer. / Brattain, Michael G.; Pretlow, Theresa P.; Pretlow, Thomas G.

In: Cancer, Vol. 40, No. 5 S, 11.1977, p. 2479-2486.

Research output: Contribution to journalArticle

Brattain, Michael G. ; Pretlow, Theresa P. ; Pretlow, Thomas G. / Cell fractionation of large bowel cancer. In: Cancer. 1977 ; Vol. 40, No. 5 S. pp. 2479-2486.
@article{ac534d43ffb147ffa7891e13fdfdca41,
title = "Cell fractionation of large bowel cancer",
abstract = "The fractionation of cells from human colonic carcinoma, a transplantable colonic carcinoma of the rat, and normal rat colonic mucosa by centrifugation in linear gradients of Ficoll (polysucrose) in tissue culture medium is described. Epithelial cells from human colonic carcinoma showed strong histochemical N‐acety‐β‐D‐glucosaminidase activity relative to stromal cells. The histochemical demonstration of this enzyme was employed to monitor the separation of malignant cells from other types of cells in human colonic carcinomas. After centrifugation, the cells from the quarter of the density gradient containing the highest percentage of cells with histochemically demonstrable N‐acetyl‐β‐D‐glucosaminidase also contained the highest levels of CEA/cell. Cells from this zone of the gradient gave rise to colonies when cultured in soft agar. Suspensions of cells from human colonic carcinomas contained eosinophils, plasma cells, lymphocytes, red blood cells and several unidentified morphological variants, which we believe to be of an epithelial nature. After centrifugation these morphological variants were located in the zone of the gradient that gave rise to colonies when cultured in soft agar. Similar morphological varieties were observed in both normal rat colonic mucosa and the transplantable rat tumor. The advantages of separated cells for a) the preparation of colonic cells for tissue culture and b) biochemical characterizations of malignant cells are described.",
author = "Brattain, {Michael G.} and Pretlow, {Theresa P.} and Pretlow, {Thomas G.}",
year = "1977",
month = "11",
doi = "10.1002/1097-0142(197711)40:5+<2479::AID-CNCR2820400914>3.0.CO;2-S",
language = "English (US)",
volume = "40",
pages = "2479--2486",
journal = "Cancer",
issn = "0008-543X",
publisher = "John Wiley and Sons Inc.",
number = "5 S",

}

TY - JOUR

T1 - Cell fractionation of large bowel cancer

AU - Brattain, Michael G.

AU - Pretlow, Theresa P.

AU - Pretlow, Thomas G.

PY - 1977/11

Y1 - 1977/11

N2 - The fractionation of cells from human colonic carcinoma, a transplantable colonic carcinoma of the rat, and normal rat colonic mucosa by centrifugation in linear gradients of Ficoll (polysucrose) in tissue culture medium is described. Epithelial cells from human colonic carcinoma showed strong histochemical N‐acety‐β‐D‐glucosaminidase activity relative to stromal cells. The histochemical demonstration of this enzyme was employed to monitor the separation of malignant cells from other types of cells in human colonic carcinomas. After centrifugation, the cells from the quarter of the density gradient containing the highest percentage of cells with histochemically demonstrable N‐acetyl‐β‐D‐glucosaminidase also contained the highest levels of CEA/cell. Cells from this zone of the gradient gave rise to colonies when cultured in soft agar. Suspensions of cells from human colonic carcinomas contained eosinophils, plasma cells, lymphocytes, red blood cells and several unidentified morphological variants, which we believe to be of an epithelial nature. After centrifugation these morphological variants were located in the zone of the gradient that gave rise to colonies when cultured in soft agar. Similar morphological varieties were observed in both normal rat colonic mucosa and the transplantable rat tumor. The advantages of separated cells for a) the preparation of colonic cells for tissue culture and b) biochemical characterizations of malignant cells are described.

AB - The fractionation of cells from human colonic carcinoma, a transplantable colonic carcinoma of the rat, and normal rat colonic mucosa by centrifugation in linear gradients of Ficoll (polysucrose) in tissue culture medium is described. Epithelial cells from human colonic carcinoma showed strong histochemical N‐acety‐β‐D‐glucosaminidase activity relative to stromal cells. The histochemical demonstration of this enzyme was employed to monitor the separation of malignant cells from other types of cells in human colonic carcinomas. After centrifugation, the cells from the quarter of the density gradient containing the highest percentage of cells with histochemically demonstrable N‐acetyl‐β‐D‐glucosaminidase also contained the highest levels of CEA/cell. Cells from this zone of the gradient gave rise to colonies when cultured in soft agar. Suspensions of cells from human colonic carcinomas contained eosinophils, plasma cells, lymphocytes, red blood cells and several unidentified morphological variants, which we believe to be of an epithelial nature. After centrifugation these morphological variants were located in the zone of the gradient that gave rise to colonies when cultured in soft agar. Similar morphological varieties were observed in both normal rat colonic mucosa and the transplantable rat tumor. The advantages of separated cells for a) the preparation of colonic cells for tissue culture and b) biochemical characterizations of malignant cells are described.

UR - http://www.scopus.com/inward/record.url?scp=0017716375&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017716375&partnerID=8YFLogxK

U2 - 10.1002/1097-0142(197711)40:5+<2479::AID-CNCR2820400914>3.0.CO;2-S

DO - 10.1002/1097-0142(197711)40:5+<2479::AID-CNCR2820400914>3.0.CO;2-S

M3 - Article

C2 - 922689

AN - SCOPUS:0017716375

VL - 40

SP - 2479

EP - 2486

JO - Cancer

JF - Cancer

SN - 0008-543X

IS - 5 S

ER -