Cell density regulates the number of cell surface receptors for fibroblast growth factor

George Veomett, Charles Kuszynski, Peter Kazakoff, Angie Rizzino

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Previous studies have shown that the binding of fibroblast growth factor to several different non-transformed cell lines decreases as cell density increases. However, it was not determined whether this is due to a reduction in receptor number or to a decrease in receptor affinity. In this study, we demonstrate that the reduction in fibroblast growth factor binding is due to a reduction in receptor number. In addition, flow cytometric analysis indicated little or no difference in the cell cycle distribution of the cells at low and high cell densities, yet the binding of fibroblast growth factor was reduced substantially at high cell densities. Thus, the reduction in growth factor binding observed in this study does not appear to result from cell cycle-dependent regulation of growth factor receptors.

Original languageEnglish (US)
Pages (from-to)694-700
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume159
Issue number2
DOIs
StatePublished - Mar 15 1989

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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