Cell density-dependent modulation of basic fibroblast growth factor expression by human interferon-β

Rakesh K. Singh, Corazon D. Bucana, Norma Llansa, Ricardo Sanchez, Isaiah J. Fidler

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The production of basic fibroblast growth factor (bFGF) by human renal cell carcinoma (HRCC) is associated with induction of angiogenesis. Incubation of HRCC cells with human interferon alpha (IFN-α) or interferon beta (IFN-β) downregulates the expression of bFGF and, hence, angiogenesis. The purpose of this study was to analyze the downregulation of the expression of bFGF in HRCC cells by IFN-α and IFN-β. Human HRCC SN12PM6 cells cultured under sparse conditions expressed 3-7-fold higher levels of steady-state bFGF-specific mRNA transcripts and cellular bFGF protein than did confluent cultures. IFN-α or IFN-β inhibited the steady-state expression of bFGF mRNA transcripts and cellular bFGF protein in a concentration-dependent manner in sparse but not confluent cultures. Moreover, IFN-β downregulated the transcription rate of bFGF genes and inhibited the de novo synthesis of bFGF protein only in sparse cultures. The results demonstrate that the inhibitory effects of IFN-α and -β on bFGF expression are cell-density dependent.

Original languageEnglish (US)
Pages (from-to)649-656
Number of pages8
JournalInternational journal of oncology
Volume8
Issue number4
Publication statusPublished - Apr 1 1996

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Keywords

  • Cell density
  • IFN-β
  • bFGF

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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