cDNA cloning and expression analysis of NeuroD mRNA in human retina

Harsha R. Acharya, Constance M. Dooley, Wallace B Thoreson, Iqbal Ahmad

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We have shown that bHLH proteins are involved in mammalian retinal development. Here we report the identification and analysis of the expression of a neurogenic differentiation gene, NeuroD, in human retina. In situ hybridization and immunocytochemical analyses of adult retina showed that NeuroD transcripts and NeuroD immunoreactivity are predominantly localized to the outer nuclear layer which contains the photoreceptors. Southern analysis of PCR-amplified cDNA revealed that NeuroD mRNA is also expressed in fetal human retina. Fetal monkey retina was used to analyse the spatial distribution of NeuroD in the developing retina. Both NeuroD transcripts and immunoreactivity are largely detected in the outer neuroblastic layer. Therefore, NeuroD may be involved in the differentiation as well as maintenance of the differentiated properties of photoreceptors.

Original languageEnglish (US)
Pages (from-to)459-463
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume233
Issue number2
DOIs
StatePublished - Apr 17 1997

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Basic Helix-Loop-Helix Transcription Factors
Cloning
Spatial distribution
Retina
Organism Cloning
Complementary DNA
Genes
Messenger RNA
Spatial Analysis
Haplorhini
In Situ Hybridization
Maintenance
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

cDNA cloning and expression analysis of NeuroD mRNA in human retina. / Acharya, Harsha R.; Dooley, Constance M.; Thoreson, Wallace B; Ahmad, Iqbal.

In: Biochemical and Biophysical Research Communications, Vol. 233, No. 2, 17.04.1997, p. 459-463.

Research output: Contribution to journalArticle

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