CD8+ cell depletion accelerates HIV-1 immunopathology in humanized mice

Santhi Gorantla, Edward Makarov, Jennifer Finke-Dwyer, Catherine L Gebhart, William Domm, Stephen Dewhurst, Howard Eliot Gendelman, Larisa Y Poluektova

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Stable engraftment of human lymphoid tissue in NOD/scid-IL- 2Rγcnull mice after CD34+ hematopoietic stem cell reconstitution permits the evaluation of ongoing HIV-1 infection for weeks to months. We demonstrate that HIV-1-infected rodents develop virus-specific cellular immune responses. CD8+ cell depletion, 2 or 5-7 wk after viral infection, resulted in a significant increase of HIV-1 load, robust immune cell activation, and cytopathology in lymphoid tissues but preserved CD4/CD8 double-positive thymic T cell pools. Human CD8+ cells reappeared in circulation as early as 2-3 wk. These data support a role of CD8+ cells in viral surveillance and the relevance of this humanized mouse model for the studies of HIV-1 pathobiology and virus-specific immunity.

Original languageEnglish (US)
Pages (from-to)7082-7091
Number of pages10
JournalJournal of Immunology
Volume184
Issue number12
DOIs
StatePublished - Jun 15 2010

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HIV-1
Lymphoid Tissue
Viruses
Virus Diseases
Hematopoietic Stem Cells
Cellular Immunity
HIV Infections
Immunity
Rodentia
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

CD8+ cell depletion accelerates HIV-1 immunopathology in humanized mice. / Gorantla, Santhi; Makarov, Edward; Finke-Dwyer, Jennifer; Gebhart, Catherine L; Domm, William; Dewhurst, Stephen; Gendelman, Howard Eliot; Poluektova, Larisa Y.

In: Journal of Immunology, Vol. 184, No. 12, 15.06.2010, p. 7082-7091.

Research output: Contribution to journalArticle

Gorantla S, Makarov E, Finke-Dwyer J, Gebhart CL, Domm W, Dewhurst S et al. CD8+ cell depletion accelerates HIV-1 immunopathology in humanized mice. Journal of Immunology. 2010 Jun 15;184(12):7082-7091. https://doi.org/10.4049/jimmunol.1000438
Gorantla, Santhi ; Makarov, Edward ; Finke-Dwyer, Jennifer ; Gebhart, Catherine L ; Domm, William ; Dewhurst, Stephen ; Gendelman, Howard Eliot ; Poluektova, Larisa Y. / CD8+ cell depletion accelerates HIV-1 immunopathology in humanized mice. In: Journal of Immunology. 2010 ; Vol. 184, No. 12. pp. 7082-7091.
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