CD44 targeted lipid nanoparticles for microRNA therapy

Stephen L. Hayward, Srivatsan S Kidambi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

MicroRNAs are small noncoding RNAs that function as powerful endogenous regulators of gene expression. Dysregulation of MicroRNA biogenesis has been correlated with the onset and progression of many human diseases. MicroRNA therapy involves the re-equilibration of aberrant intracellular MicroRNA expression profiles for long-term disease management. Despite the significant potential of MicroRNA therapy, the utilization of MicroRNA-based therapeutics has been drastically hindered in practice by the lack of a targeted and translatable delivery vehicle. CD44 is a cell surface glycoprotein that facilitates cellular communication and motility through cell-cell and cell-extracellular matrix interactions. CD44 has been shown to be elevated in multiple disease states including cancer making it a potential diagnostic biomarker and an ideal receptor for targeted drug delivery systems. We describe a method for targeting CD44 using a lipid nanocarrier for the cytoplasmic delivery of active MicroRNA.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages95-109
Number of pages15
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1831
ISSN (Print)1064-3745

Fingerprint

MicroRNAs
Nanoparticles
Lipids
Therapeutics
Small Untranslated RNA
Membrane Glycoproteins
Drug Delivery Systems
Regulator Genes
Disease Management
Cell Movement
Extracellular Matrix
Biomarkers
Communication
Gene Expression
Neoplasms

Keywords

  • Active targeting
  • CD44
  • Gene therapy
  • Hyaluronic acid
  • Lipid nanoparticles
  • Liposomes
  • MicroRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Hayward, S. L., & Kidambi, S. S. (2018). CD44 targeted lipid nanoparticles for microRNA therapy. In Methods in Molecular Biology (pp. 95-109). (Methods in Molecular Biology; Vol. 1831). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8661-3_8

CD44 targeted lipid nanoparticles for microRNA therapy. / Hayward, Stephen L.; Kidambi, Srivatsan S.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 95-109 (Methods in Molecular Biology; Vol. 1831).

Research output: Chapter in Book/Report/Conference proceedingChapter

Hayward, SL & Kidambi, SS 2018, CD44 targeted lipid nanoparticles for microRNA therapy. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1831, Humana Press Inc., pp. 95-109. https://doi.org/10.1007/978-1-4939-8661-3_8
Hayward SL, Kidambi SS. CD44 targeted lipid nanoparticles for microRNA therapy. In Methods in Molecular Biology. Humana Press Inc. 2018. p. 95-109. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8661-3_8
Hayward, Stephen L. ; Kidambi, Srivatsan S. / CD44 targeted lipid nanoparticles for microRNA therapy. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 95-109 (Methods in Molecular Biology).
@inbook{c1b6c74166e34098a6e83b9eb57665e7,
title = "CD44 targeted lipid nanoparticles for microRNA therapy",
abstract = "MicroRNAs are small noncoding RNAs that function as powerful endogenous regulators of gene expression. Dysregulation of MicroRNA biogenesis has been correlated with the onset and progression of many human diseases. MicroRNA therapy involves the re-equilibration of aberrant intracellular MicroRNA expression profiles for long-term disease management. Despite the significant potential of MicroRNA therapy, the utilization of MicroRNA-based therapeutics has been drastically hindered in practice by the lack of a targeted and translatable delivery vehicle. CD44 is a cell surface glycoprotein that facilitates cellular communication and motility through cell-cell and cell-extracellular matrix interactions. CD44 has been shown to be elevated in multiple disease states including cancer making it a potential diagnostic biomarker and an ideal receptor for targeted drug delivery systems. We describe a method for targeting CD44 using a lipid nanocarrier for the cytoplasmic delivery of active MicroRNA.",
keywords = "Active targeting, CD44, Gene therapy, Hyaluronic acid, Lipid nanoparticles, Liposomes, MicroRNA",
author = "Hayward, {Stephen L.} and Kidambi, {Srivatsan S}",
year = "2018",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-8661-3_8",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "95--109",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - CD44 targeted lipid nanoparticles for microRNA therapy

AU - Hayward, Stephen L.

AU - Kidambi, Srivatsan S

PY - 2018/1/1

Y1 - 2018/1/1

N2 - MicroRNAs are small noncoding RNAs that function as powerful endogenous regulators of gene expression. Dysregulation of MicroRNA biogenesis has been correlated with the onset and progression of many human diseases. MicroRNA therapy involves the re-equilibration of aberrant intracellular MicroRNA expression profiles for long-term disease management. Despite the significant potential of MicroRNA therapy, the utilization of MicroRNA-based therapeutics has been drastically hindered in practice by the lack of a targeted and translatable delivery vehicle. CD44 is a cell surface glycoprotein that facilitates cellular communication and motility through cell-cell and cell-extracellular matrix interactions. CD44 has been shown to be elevated in multiple disease states including cancer making it a potential diagnostic biomarker and an ideal receptor for targeted drug delivery systems. We describe a method for targeting CD44 using a lipid nanocarrier for the cytoplasmic delivery of active MicroRNA.

AB - MicroRNAs are small noncoding RNAs that function as powerful endogenous regulators of gene expression. Dysregulation of MicroRNA biogenesis has been correlated with the onset and progression of many human diseases. MicroRNA therapy involves the re-equilibration of aberrant intracellular MicroRNA expression profiles for long-term disease management. Despite the significant potential of MicroRNA therapy, the utilization of MicroRNA-based therapeutics has been drastically hindered in practice by the lack of a targeted and translatable delivery vehicle. CD44 is a cell surface glycoprotein that facilitates cellular communication and motility through cell-cell and cell-extracellular matrix interactions. CD44 has been shown to be elevated in multiple disease states including cancer making it a potential diagnostic biomarker and an ideal receptor for targeted drug delivery systems. We describe a method for targeting CD44 using a lipid nanocarrier for the cytoplasmic delivery of active MicroRNA.

KW - Active targeting

KW - CD44

KW - Gene therapy

KW - Hyaluronic acid

KW - Lipid nanoparticles

KW - Liposomes

KW - MicroRNA

UR - http://www.scopus.com/inward/record.url?scp=85051237575&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85051237575&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-8661-3_8

DO - 10.1007/978-1-4939-8661-3_8

M3 - Chapter

T3 - Methods in Molecular Biology

SP - 95

EP - 109

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -