CD44 in normal human pancreas and pancreatic carcinoma cell lines

Jörg Ringel, Ralf Jesnowski, Christian Schmidt, Jens Ringel, Hans J. Köhler, Joachim Rychly, Surinder Kumar Batra, Matthias Löhr

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

CD44 is an integral cell-surface glycoprotein. Overexpression of the CD44 standard (CD44st) and its variants (CD44v) has been implicated in transformation and progression of many cancer types. Here, we investigated expression of CD44st, CD44v3-7, CD44v7/8, and v10 in five human pancreatic tumor cell lines and normal human pancreatic duct cells transfected with the SV40 large T antigen. CD44st and its variant proteins were quantified using immunocytochemistry and flow cytometry. CD44v7 was expressed at low levels, whereas CD44st, CD44v3, CD44v4, CD44v, and CD44v6 were expressed at moderate levels in all pancreatic tumor cell lines. In contrast, CD44v7/8 and CD44v10 were expressed at very low levels in two out of the five pancreatic tumor cell lines. Overall, staining of CD44st and CD44 variants was significantly weaker compared to another surface molecule, ICAM-1, reported to be overexpressed in pancreatic cancer cells. Furthermore, the SV40 large T transfected duct cells showed only a weak staining for CD44st, CD44v5, and CD44v6. To determine a possible mechanism for the regulation of surface expression of CD44st, v5 and v6, we incubated Panc-1 cells with bFGF, TGF-β1, EGF, TNFα, and IFNγ. Only IFNγ affected the CD44 expression by downregulation of CD44v6. The constitutive expression of CD44 variants seems to be associated with the malignant state of invasive carcinoma.

Original languageEnglish (US)
Pages (from-to)97-106
Number of pages10
JournalTeratogenesis Carcinogenesis and Mutagenesis
Volume21
Issue number1
DOIs
StatePublished - Jan 1 2001

Fingerprint

Pancreas
Cells
Cell Line
Tumor Cell Line
Tumors
Ducts
Staining and Labeling
Polyomavirus Transforming Antigens
Flow cytometry
Pancreatic Ducts
Viral Tumor Antigens
Membrane Glycoproteins
Intercellular Adhesion Molecule-1
Pancreatic Carcinoma
Pancreatic Neoplasms
Epidermal Growth Factor
Flow Cytometry
Down-Regulation
Immunohistochemistry
Carcinoma

Keywords

  • CD44
  • Growth factors
  • Pancreatic adenocarcinoma
  • Pancreatic cancer cell lines

ASJC Scopus subject areas

  • Oncology
  • Genetics
  • Toxicology
  • Genetics(clinical)
  • Health, Toxicology and Mutagenesis

Cite this

Ringel, J., Jesnowski, R., Schmidt, C., Ringel, J., Köhler, H. J., Rychly, J., ... Löhr, M. (2001). CD44 in normal human pancreas and pancreatic carcinoma cell lines. Teratogenesis Carcinogenesis and Mutagenesis, 21(1), 97-106. https://doi.org/10.1002/1520-6866(2001)21:1<97::AID-TCM9>3.0.CO;2-O

CD44 in normal human pancreas and pancreatic carcinoma cell lines. / Ringel, Jörg; Jesnowski, Ralf; Schmidt, Christian; Ringel, Jens; Köhler, Hans J.; Rychly, Joachim; Batra, Surinder Kumar; Löhr, Matthias.

In: Teratogenesis Carcinogenesis and Mutagenesis, Vol. 21, No. 1, 01.01.2001, p. 97-106.

Research output: Contribution to journalArticle

Ringel, Jörg ; Jesnowski, Ralf ; Schmidt, Christian ; Ringel, Jens ; Köhler, Hans J. ; Rychly, Joachim ; Batra, Surinder Kumar ; Löhr, Matthias. / CD44 in normal human pancreas and pancreatic carcinoma cell lines. In: Teratogenesis Carcinogenesis and Mutagenesis. 2001 ; Vol. 21, No. 1. pp. 97-106.
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