CD43 is a murine T cell costimulatory receptor that functions independently of CD28

Anne I. Sperling, Jonathan M. Green, R Lee Mosley, Peter L. Smith, Richard J. DiPaolo, John R. Klein, Jeffrey A. Bluestone, Craig B. Thompson

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

Costimulation mediated by the CD28 receptor has been shown to play an important role in the development of a vigorous T cell immune response. Nevertheless, CD28-deficient mice can mount effective T cell-dependent immune responses. These data suggest that other costimulatory molecules may play a role in T cell activation. In a search for other costimulatory receptors on T ceils, we have characterized a monoclonal antibody (mAb) that can costimulate T cells in the absence of accessory cells. Similar to CD28 antibodies, this mAb, R2/60, was found to synergize with T cell receptor engagement in inducing proliferation. Independent ligation of CD3 and the ligand recognized by R2/60 results in T cell proliferation, suggesting that the two molecules do not have to colocalize to activate the R2/60 costimulatory pathway. R2/60 does not react with CD28, and furthermore, R2/60 costimulates in a CD28-independent fashion since the mAb costimulates T cells from the CD28-deficient mice as well as wild-type mice. Expression doning of the R2/60 antigen identified the ligand as murine CD43. Together, these data demonstrate that CD43 can serve as a receptor on T cells that can provide CD28-independent costimulation.

Original languageEnglish (US)
Pages (from-to)139-146
Number of pages8
JournalJournal of Experimental Medicine
Volume182
Issue number1
DOIs
StatePublished - Jul 1 1995

Fingerprint

Costimulatory and Inhibitory T-Cell Receptors
T-Lymphocytes
Monoclonal Antibodies
T-Cell Antigen Receptor
Ligands
Ligation
Cell Proliferation
Antigens
Antibodies

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Sperling, A. I., Green, J. M., Mosley, R. L., Smith, P. L., DiPaolo, R. J., Klein, J. R., ... Thompson, C. B. (1995). CD43 is a murine T cell costimulatory receptor that functions independently of CD28. Journal of Experimental Medicine, 182(1), 139-146. https://doi.org/10.1084/jem.182.1.139

CD43 is a murine T cell costimulatory receptor that functions independently of CD28. / Sperling, Anne I.; Green, Jonathan M.; Mosley, R Lee; Smith, Peter L.; DiPaolo, Richard J.; Klein, John R.; Bluestone, Jeffrey A.; Thompson, Craig B.

In: Journal of Experimental Medicine, Vol. 182, No. 1, 01.07.1995, p. 139-146.

Research output: Contribution to journalArticle

Sperling, AI, Green, JM, Mosley, RL, Smith, PL, DiPaolo, RJ, Klein, JR, Bluestone, JA & Thompson, CB 1995, 'CD43 is a murine T cell costimulatory receptor that functions independently of CD28', Journal of Experimental Medicine, vol. 182, no. 1, pp. 139-146. https://doi.org/10.1084/jem.182.1.139
Sperling, Anne I. ; Green, Jonathan M. ; Mosley, R Lee ; Smith, Peter L. ; DiPaolo, Richard J. ; Klein, John R. ; Bluestone, Jeffrey A. ; Thompson, Craig B. / CD43 is a murine T cell costimulatory receptor that functions independently of CD28. In: Journal of Experimental Medicine. 1995 ; Vol. 182, No. 1. pp. 139-146.
@article{0cede812c5ab4df4a4804391b91ab863,
title = "CD43 is a murine T cell costimulatory receptor that functions independently of CD28",
abstract = "Costimulation mediated by the CD28 receptor has been shown to play an important role in the development of a vigorous T cell immune response. Nevertheless, CD28-deficient mice can mount effective T cell-dependent immune responses. These data suggest that other costimulatory molecules may play a role in T cell activation. In a search for other costimulatory receptors on T ceils, we have characterized a monoclonal antibody (mAb) that can costimulate T cells in the absence of accessory cells. Similar to CD28 antibodies, this mAb, R2/60, was found to synergize with T cell receptor engagement in inducing proliferation. Independent ligation of CD3 and the ligand recognized by R2/60 results in T cell proliferation, suggesting that the two molecules do not have to colocalize to activate the R2/60 costimulatory pathway. R2/60 does not react with CD28, and furthermore, R2/60 costimulates in a CD28-independent fashion since the mAb costimulates T cells from the CD28-deficient mice as well as wild-type mice. Expression doning of the R2/60 antigen identified the ligand as murine CD43. Together, these data demonstrate that CD43 can serve as a receptor on T cells that can provide CD28-independent costimulation.",
author = "Sperling, {Anne I.} and Green, {Jonathan M.} and Mosley, {R Lee} and Smith, {Peter L.} and DiPaolo, {Richard J.} and Klein, {John R.} and Bluestone, {Jeffrey A.} and Thompson, {Craig B.}",
year = "1995",
month = "7",
day = "1",
doi = "10.1084/jem.182.1.139",
language = "English (US)",
volume = "182",
pages = "139--146",
journal = "Journal of Experimental Medicine",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "1",

}

TY - JOUR

T1 - CD43 is a murine T cell costimulatory receptor that functions independently of CD28

AU - Sperling, Anne I.

AU - Green, Jonathan M.

AU - Mosley, R Lee

AU - Smith, Peter L.

AU - DiPaolo, Richard J.

AU - Klein, John R.

AU - Bluestone, Jeffrey A.

AU - Thompson, Craig B.

PY - 1995/7/1

Y1 - 1995/7/1

N2 - Costimulation mediated by the CD28 receptor has been shown to play an important role in the development of a vigorous T cell immune response. Nevertheless, CD28-deficient mice can mount effective T cell-dependent immune responses. These data suggest that other costimulatory molecules may play a role in T cell activation. In a search for other costimulatory receptors on T ceils, we have characterized a monoclonal antibody (mAb) that can costimulate T cells in the absence of accessory cells. Similar to CD28 antibodies, this mAb, R2/60, was found to synergize with T cell receptor engagement in inducing proliferation. Independent ligation of CD3 and the ligand recognized by R2/60 results in T cell proliferation, suggesting that the two molecules do not have to colocalize to activate the R2/60 costimulatory pathway. R2/60 does not react with CD28, and furthermore, R2/60 costimulates in a CD28-independent fashion since the mAb costimulates T cells from the CD28-deficient mice as well as wild-type mice. Expression doning of the R2/60 antigen identified the ligand as murine CD43. Together, these data demonstrate that CD43 can serve as a receptor on T cells that can provide CD28-independent costimulation.

AB - Costimulation mediated by the CD28 receptor has been shown to play an important role in the development of a vigorous T cell immune response. Nevertheless, CD28-deficient mice can mount effective T cell-dependent immune responses. These data suggest that other costimulatory molecules may play a role in T cell activation. In a search for other costimulatory receptors on T ceils, we have characterized a monoclonal antibody (mAb) that can costimulate T cells in the absence of accessory cells. Similar to CD28 antibodies, this mAb, R2/60, was found to synergize with T cell receptor engagement in inducing proliferation. Independent ligation of CD3 and the ligand recognized by R2/60 results in T cell proliferation, suggesting that the two molecules do not have to colocalize to activate the R2/60 costimulatory pathway. R2/60 does not react with CD28, and furthermore, R2/60 costimulates in a CD28-independent fashion since the mAb costimulates T cells from the CD28-deficient mice as well as wild-type mice. Expression doning of the R2/60 antigen identified the ligand as murine CD43. Together, these data demonstrate that CD43 can serve as a receptor on T cells that can provide CD28-independent costimulation.

UR - http://www.scopus.com/inward/record.url?scp=0029065528&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029065528&partnerID=8YFLogxK

U2 - 10.1084/jem.182.1.139

DO - 10.1084/jem.182.1.139

M3 - Article

VL - 182

SP - 139

EP - 146

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

SN - 0022-1007

IS - 1

ER -