CD226 is specifically expressed on the surface of Th1 cells and regulates their expansion and effector functions

Valerie Dardalhon, Anna S. Schubart, Jayagopala Reddy, Jennifer Hartt Meyers, Laurent Monney, Catherine A. Sabatos, Rakesh Ahuja, Khuong Nguyen, Gordon J. Freeman, Edward A. Greenfield, Raymond A. Sobel, Vijay K. Kuchroo

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Surface molecules that are differentially expressed on Th1 and Th2 cells may be useful in regulating specific immune responses in vivo. Using a panel of mAbs, we have identified murine CD226 as specifically expressed on the surface of differentiated Th1 cells but not Th2 or Th0 cells. Although CD226 is constitutively expressed on CD8 cells, it is up-regulated on CD4 cells upon activation. Th1 differentiation results in enhanced CD226 expression, whereas expression is down-regulated upon Th2 polarization. We demonstrate that CD226 is involved in the regulation of T cell activation; in vivo treatment with anti-CD226 results in significant reduction of Th1 cell expansion and in the induction of APCs that inhibit T cell activation. Furthermore, anti-CD226 treatment delays the onset and reduces the severity of a Th1-mediated autoimmune disease, experimental autoimmune encephalomyelitis. Our data suggest that CD226 is a costimulatory molecule that plays an important role in activation and effector functions of Th1 cells.

Original languageEnglish (US)
Pages (from-to)1558-1565
Number of pages8
JournalJournal of Immunology
Volume175
Issue number3
DOIs
StatePublished - Aug 1 2005

Fingerprint

Th1 Cells
T-Lymphocytes
Th2 Cells
Autoimmune Experimental Encephalomyelitis
Autoimmune Diseases

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Dardalhon, V., Schubart, A. S., Reddy, J., Meyers, J. H., Monney, L., Sabatos, C. A., ... Kuchroo, V. K. (2005). CD226 is specifically expressed on the surface of Th1 cells and regulates their expansion and effector functions. Journal of Immunology, 175(3), 1558-1565. https://doi.org/10.4049/jimmunol.175.3.1558

CD226 is specifically expressed on the surface of Th1 cells and regulates their expansion and effector functions. / Dardalhon, Valerie; Schubart, Anna S.; Reddy, Jayagopala; Meyers, Jennifer Hartt; Monney, Laurent; Sabatos, Catherine A.; Ahuja, Rakesh; Nguyen, Khuong; Freeman, Gordon J.; Greenfield, Edward A.; Sobel, Raymond A.; Kuchroo, Vijay K.

In: Journal of Immunology, Vol. 175, No. 3, 01.08.2005, p. 1558-1565.

Research output: Contribution to journalArticle

Dardalhon, V, Schubart, AS, Reddy, J, Meyers, JH, Monney, L, Sabatos, CA, Ahuja, R, Nguyen, K, Freeman, GJ, Greenfield, EA, Sobel, RA & Kuchroo, VK 2005, 'CD226 is specifically expressed on the surface of Th1 cells and regulates their expansion and effector functions', Journal of Immunology, vol. 175, no. 3, pp. 1558-1565. https://doi.org/10.4049/jimmunol.175.3.1558
Dardalhon, Valerie ; Schubart, Anna S. ; Reddy, Jayagopala ; Meyers, Jennifer Hartt ; Monney, Laurent ; Sabatos, Catherine A. ; Ahuja, Rakesh ; Nguyen, Khuong ; Freeman, Gordon J. ; Greenfield, Edward A. ; Sobel, Raymond A. ; Kuchroo, Vijay K. / CD226 is specifically expressed on the surface of Th1 cells and regulates their expansion and effector functions. In: Journal of Immunology. 2005 ; Vol. 175, No. 3. pp. 1558-1565.
@article{b6be0806e8494fcf9a726e0742b16b73,
title = "CD226 is specifically expressed on the surface of Th1 cells and regulates their expansion and effector functions",
abstract = "Surface molecules that are differentially expressed on Th1 and Th2 cells may be useful in regulating specific immune responses in vivo. Using a panel of mAbs, we have identified murine CD226 as specifically expressed on the surface of differentiated Th1 cells but not Th2 or Th0 cells. Although CD226 is constitutively expressed on CD8 cells, it is up-regulated on CD4 cells upon activation. Th1 differentiation results in enhanced CD226 expression, whereas expression is down-regulated upon Th2 polarization. We demonstrate that CD226 is involved in the regulation of T cell activation; in vivo treatment with anti-CD226 results in significant reduction of Th1 cell expansion and in the induction of APCs that inhibit T cell activation. Furthermore, anti-CD226 treatment delays the onset and reduces the severity of a Th1-mediated autoimmune disease, experimental autoimmune encephalomyelitis. Our data suggest that CD226 is a costimulatory molecule that plays an important role in activation and effector functions of Th1 cells.",
author = "Valerie Dardalhon and Schubart, {Anna S.} and Jayagopala Reddy and Meyers, {Jennifer Hartt} and Laurent Monney and Sabatos, {Catherine A.} and Rakesh Ahuja and Khuong Nguyen and Freeman, {Gordon J.} and Greenfield, {Edward A.} and Sobel, {Raymond A.} and Kuchroo, {Vijay K.}",
year = "2005",
month = "8",
day = "1",
doi = "10.4049/jimmunol.175.3.1558",
language = "English (US)",
volume = "175",
pages = "1558--1565",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "3",

}

TY - JOUR

T1 - CD226 is specifically expressed on the surface of Th1 cells and regulates their expansion and effector functions

AU - Dardalhon, Valerie

AU - Schubart, Anna S.

AU - Reddy, Jayagopala

AU - Meyers, Jennifer Hartt

AU - Monney, Laurent

AU - Sabatos, Catherine A.

AU - Ahuja, Rakesh

AU - Nguyen, Khuong

AU - Freeman, Gordon J.

AU - Greenfield, Edward A.

AU - Sobel, Raymond A.

AU - Kuchroo, Vijay K.

PY - 2005/8/1

Y1 - 2005/8/1

N2 - Surface molecules that are differentially expressed on Th1 and Th2 cells may be useful in regulating specific immune responses in vivo. Using a panel of mAbs, we have identified murine CD226 as specifically expressed on the surface of differentiated Th1 cells but not Th2 or Th0 cells. Although CD226 is constitutively expressed on CD8 cells, it is up-regulated on CD4 cells upon activation. Th1 differentiation results in enhanced CD226 expression, whereas expression is down-regulated upon Th2 polarization. We demonstrate that CD226 is involved in the regulation of T cell activation; in vivo treatment with anti-CD226 results in significant reduction of Th1 cell expansion and in the induction of APCs that inhibit T cell activation. Furthermore, anti-CD226 treatment delays the onset and reduces the severity of a Th1-mediated autoimmune disease, experimental autoimmune encephalomyelitis. Our data suggest that CD226 is a costimulatory molecule that plays an important role in activation and effector functions of Th1 cells.

AB - Surface molecules that are differentially expressed on Th1 and Th2 cells may be useful in regulating specific immune responses in vivo. Using a panel of mAbs, we have identified murine CD226 as specifically expressed on the surface of differentiated Th1 cells but not Th2 or Th0 cells. Although CD226 is constitutively expressed on CD8 cells, it is up-regulated on CD4 cells upon activation. Th1 differentiation results in enhanced CD226 expression, whereas expression is down-regulated upon Th2 polarization. We demonstrate that CD226 is involved in the regulation of T cell activation; in vivo treatment with anti-CD226 results in significant reduction of Th1 cell expansion and in the induction of APCs that inhibit T cell activation. Furthermore, anti-CD226 treatment delays the onset and reduces the severity of a Th1-mediated autoimmune disease, experimental autoimmune encephalomyelitis. Our data suggest that CD226 is a costimulatory molecule that plays an important role in activation and effector functions of Th1 cells.

UR - http://www.scopus.com/inward/record.url?scp=22544455033&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=22544455033&partnerID=8YFLogxK

U2 - 10.4049/jimmunol.175.3.1558

DO - 10.4049/jimmunol.175.3.1558

M3 - Article

C2 - 16034094

AN - SCOPUS:22544455033

VL - 175

SP - 1558

EP - 1565

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 3

ER -