CcpA coordinates central metabolism and biofilm formation in Staphylococcus epidermidis

Marat R. Sadykov, Torsten Hartmann, Theodoric A. Mattes, Megan Hiatt, Naja J. Jann, Yefei Zhu, Nagender Ledala, Regine Landmann, Mathias Herrmann, Holger Rohde, Markus Bischoff, Greg A Somerville

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Staphylococcus epidermidis is an opportunistic bacterium whose infections often involve the formation of a biofilm on implanted biomaterials. In S. epidermidis, the exopolysaccharide facilitating bacterial adherence in a biofilm is polysaccharide intercellular adhesin (PIA), whose synthesis requires the enzymes encoded within the intercellular adhesin operon (icaADBC). In vitro, the formation of S. epidermidis biofilms is enhanced by conditions that repress tricarboxylic acid (TCA) cycle activity, such as growth in a medium containing glucose. In many Gram-positive bacteria, repression of TCA cycle genes in response to glucose is accomplished by catabolite control protein A (CcpA). CcpA is a member of the GalR-LacI repressor family that mediates carbon catabolite repression, leading us to hypothesize that catabolite control of S. epidermidis biofilm formation is indirectly regulated by CcpA-dependent repression of the TCA cycle. To test this hypothesis, ccpA deletion mutants were constructed in strain 1457 and 1457-acnA and the effects on TCA cycle activity, biofilm formation and virulence were assessed. As anticipated, deletion of ccpA derepressed TCA cycle activity and inhibited biofilm formation; however, ccpA deletion had only a modest effect on icaADBC transcription. Surprisingly, deletion of ccpA in strain 1457-acnA, a strain whose TCA cycle is inactive and where icaADBC transcription is derepressed, strongly inhibited icaADBC transcription. These observations demonstrate that CcpA is a positive effector of biofilm formation and icaADBC transcription and a repressor of TCA cycle activity.

Original languageEnglish (US)
Pages (from-to)3458-3468
Number of pages11
JournalMicrobiology
Volume157
Issue number12
DOIs
StatePublished - Dec 1 2011

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Citric Acid Cycle
Staphylococcus epidermidis
Staphylococcal Protein A
Biofilms
Catabolite Repression
Glucose
Gram-Positive Bacteria
Biocompatible Materials
Operon
Virulence
Bacteria
Enzymes
Growth
Infection
Genes

ASJC Scopus subject areas

  • Microbiology

Cite this

Sadykov, M. R., Hartmann, T., Mattes, T. A., Hiatt, M., Jann, N. J., Zhu, Y., ... Somerville, G. A. (2011). CcpA coordinates central metabolism and biofilm formation in Staphylococcus epidermidis. Microbiology, 157(12), 3458-3468. https://doi.org/10.1099/mic.0.051243-0

CcpA coordinates central metabolism and biofilm formation in Staphylococcus epidermidis. / Sadykov, Marat R.; Hartmann, Torsten; Mattes, Theodoric A.; Hiatt, Megan; Jann, Naja J.; Zhu, Yefei; Ledala, Nagender; Landmann, Regine; Herrmann, Mathias; Rohde, Holger; Bischoff, Markus; Somerville, Greg A.

In: Microbiology, Vol. 157, No. 12, 01.12.2011, p. 3458-3468.

Research output: Contribution to journalArticle

Sadykov, MR, Hartmann, T, Mattes, TA, Hiatt, M, Jann, NJ, Zhu, Y, Ledala, N, Landmann, R, Herrmann, M, Rohde, H, Bischoff, M & Somerville, GA 2011, 'CcpA coordinates central metabolism and biofilm formation in Staphylococcus epidermidis', Microbiology, vol. 157, no. 12, pp. 3458-3468. https://doi.org/10.1099/mic.0.051243-0
Sadykov MR, Hartmann T, Mattes TA, Hiatt M, Jann NJ, Zhu Y et al. CcpA coordinates central metabolism and biofilm formation in Staphylococcus epidermidis. Microbiology. 2011 Dec 1;157(12):3458-3468. https://doi.org/10.1099/mic.0.051243-0
Sadykov, Marat R. ; Hartmann, Torsten ; Mattes, Theodoric A. ; Hiatt, Megan ; Jann, Naja J. ; Zhu, Yefei ; Ledala, Nagender ; Landmann, Regine ; Herrmann, Mathias ; Rohde, Holger ; Bischoff, Markus ; Somerville, Greg A. / CcpA coordinates central metabolism and biofilm formation in Staphylococcus epidermidis. In: Microbiology. 2011 ; Vol. 157, No. 12. pp. 3458-3468.
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AU - Zhu, Yefei

AU - Ledala, Nagender

AU - Landmann, Regine

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