CcpA affects infectivity of Staphylococcus aureus in a hyperglycemic environment

Markus Bischoff, Bodo Wonnenberg, Nadine Nippe, Naja J. Nyffenegger-Jann, Meike Voss, Christoph Beisswenger, Cord Sunderkötter, Virginie Molle, Quoc Thai Dinh, Frank Lammert, Robert Bals, Mathias Herrmann, Greg A Somerville, Thomas Tschernig, Rosmarie Gaupp

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Many bacteria regulate the expression of virulence factors via carbon catabolite responsive elements. In Gram-positive bacteria, the predominant mediator of carbon catabolite repression is the catabolite control protein A (CcpA). Hyperglycemia is a widespread disorder that predisposes individuals to an array of symptoms and an increased risk of infections. In hyperglycemic individuals, the bacterium Staphylococcus aureus causes serious, life-threatening infections. The importance of CcpA in regulating carbon catabolite repression in S. aureus suggests it may be important for infections in hyperglycemic individuals. To test this suggestion, hyperglycemic non-obese diabetic (NOD; blood glucose level ≥20 mM) mice were challenged with the mouse pathogenic S. aureus strain Newman and the isogenic ccpA deletion mutant (MST14), and the effects on infectivity were determined. Diabetic NOD mice challenged with the ccpA deletion mutant enhanced the symptoms of infection in an acute murine pneumonia model relative to the parental strain. Interestingly, when diabetic NOD mice were used in footpad or catheter infection models, infectivity of the ccpA mutant decreased relative to the parental strain. These differences greatly diminished when normoglycemic NOD mice (blood glucose level ≤10 mM) were used. These data suggest that CcpA is important for infectivity of S. aureus in hyperglycemic individuals.

Original languageEnglish (US)
Article number172
JournalFrontiers in Cellular and Infection Microbiology
Volume7
Issue numberMAY
DOIs
StatePublished - May 9 2017

Fingerprint

Staphylococcal Protein A
Staphylococcus aureus
Inbred NOD Mouse
Catabolite Repression
Infection
Blood Glucose
Bacteria
Gram-Positive Bacteria
Virulence Factors
Hyperglycemia
Pneumonia
Carbon
Catheters

Keywords

  • Carbon catabolic regulation
  • CcpA
  • Hyperglycemia
  • Infectivity
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Bischoff, M., Wonnenberg, B., Nippe, N., Nyffenegger-Jann, N. J., Voss, M., Beisswenger, C., ... Gaupp, R. (2017). CcpA affects infectivity of Staphylococcus aureus in a hyperglycemic environment. Frontiers in Cellular and Infection Microbiology, 7(MAY), [172]. https://doi.org/10.3389/fcimb.2017.00172

CcpA affects infectivity of Staphylococcus aureus in a hyperglycemic environment. / Bischoff, Markus; Wonnenberg, Bodo; Nippe, Nadine; Nyffenegger-Jann, Naja J.; Voss, Meike; Beisswenger, Christoph; Sunderkötter, Cord; Molle, Virginie; Dinh, Quoc Thai; Lammert, Frank; Bals, Robert; Herrmann, Mathias; Somerville, Greg A; Tschernig, Thomas; Gaupp, Rosmarie.

In: Frontiers in Cellular and Infection Microbiology, Vol. 7, No. MAY, 172, 09.05.2017.

Research output: Contribution to journalArticle

Bischoff, M, Wonnenberg, B, Nippe, N, Nyffenegger-Jann, NJ, Voss, M, Beisswenger, C, Sunderkötter, C, Molle, V, Dinh, QT, Lammert, F, Bals, R, Herrmann, M, Somerville, GA, Tschernig, T & Gaupp, R 2017, 'CcpA affects infectivity of Staphylococcus aureus in a hyperglycemic environment', Frontiers in Cellular and Infection Microbiology, vol. 7, no. MAY, 172. https://doi.org/10.3389/fcimb.2017.00172
Bischoff M, Wonnenberg B, Nippe N, Nyffenegger-Jann NJ, Voss M, Beisswenger C et al. CcpA affects infectivity of Staphylococcus aureus in a hyperglycemic environment. Frontiers in Cellular and Infection Microbiology. 2017 May 9;7(MAY). 172. https://doi.org/10.3389/fcimb.2017.00172
Bischoff, Markus ; Wonnenberg, Bodo ; Nippe, Nadine ; Nyffenegger-Jann, Naja J. ; Voss, Meike ; Beisswenger, Christoph ; Sunderkötter, Cord ; Molle, Virginie ; Dinh, Quoc Thai ; Lammert, Frank ; Bals, Robert ; Herrmann, Mathias ; Somerville, Greg A ; Tschernig, Thomas ; Gaupp, Rosmarie. / CcpA affects infectivity of Staphylococcus aureus in a hyperglycemic environment. In: Frontiers in Cellular and Infection Microbiology. 2017 ; Vol. 7, No. MAY.
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