CCND2 and CCND3 hijack immunoglobulin light-chain enhancers in cyclin D12 mantle cell lymphoma

David Martín-Garcia, Alba Navarro, Rafael Valdés-Mas, Guillem Clot, Jesús Gutiérrez-Abril, Miriam Prieto, Inmaculada Ribera-Cortada, Renata Woroniecka, Grzegorz Rymkiewicz, Susanne Bens, Laurence De Leval, Andreas Rosenwald, Judith A. Ferry, Eric D. Hsi, Kai Fu, Jan Delabie, Dennis Weisenburger, Daphne De Jong, Fina Climent, Sheila J. O'ConnorSteven H. Swerdlow, David Torrents, Sergi Beltran, Blanca Espinet, Blanca González-Farre, Luis Veloza, Dolors Costa, Estella Matutes, Reiner Siebert, German Ott, Leticia Quintanilla-Martinez, Elaine S. Jaffe, Carlos López-Otín, Itziar Salaverria, Xose S. Puente, Elias Campo, Silvia Bea

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Mantle cell lymphoma (MCL) is characterized by the t(11;14)(q13;q32) translocation resulting in overexpression of cyclin D1. However, a small subset of cyclin D12 MCL has been recognized, and approximately one-half of them harbor CCND2 translocations while the primary event in cyclinD12/D22MCL remains elusive. To identify other potentialmechanisms driving MCL pathogenesis, we investigated 56 cyclin D12/SOX111 MCL by fluorescence in situ hybridization (FISH), whole-genome/exome sequencing, and gene-expression and copy-number arrays. FISH with break-apart probes identified CCND2 rearrangements in 39 cases (70%) but not CCND3 rearrangements. We analyzed 3 of these negative cases by whole-genome/exome sequencing and identified IGK (n 5 2) and IGL (n 5 1) enhancer hijackings near CCND3 that were associated with cyclin D3 overexpression. By specific FISH probes, including the IGK enhancer region, we detected 10 additional cryptic IGK juxtapositions to CCND3 (6 cases) and CCND2 (4 cases) in MCL that overexpressed, respectively, these cyclins. A minor subset of 4 cyclin D12 MCL cases lacked cyclin D rearrangements and showed upregulation of CCNE1 and CCNE2. These cases had blastoid morphology, high genomic complexity, and CDKN2A and RB1 deletions. Both genomic and gene-expression profiles of cyclin D12 MCL cases were indistinguishable from cyclin D11 MCL. In conclusion, virtually all cyclin D12 MCLs carry CCND2/CCND3 rearrangements with immunoglobulin genes, including a novel IGK/L enhancer hijacking mechanism. A subset of cyclin D12/D22/D32 MCL with aggressive features has cyclin E dysregulation. Specific FISH probes may allow the molecular identification and diagnosis of cyclin D12 MCL.

Original languageEnglish (US)
Pages (from-to)940-951
Number of pages12
JournalBlood
Volume133
Issue number9
DOIs
StatePublished - Jan 1 2019

Fingerprint

Immunoglobulin Light Chains
Mantle-Cell Lymphoma
Cyclins
Fluorescence In Situ Hybridization
Fluorescence
Exome
Genes
Gene expression
Cyclin D3
Cyclin D
Genome
Cyclin E
Cyclin D1
Immunoglobulin Genes
Gene Dosage
Ports and harbors
Transcriptome
Immunoglobulins
Up-Regulation

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Martín-Garcia, D., Navarro, A., Valdés-Mas, R., Clot, G., Gutiérrez-Abril, J., Prieto, M., ... Bea, S. (2019). CCND2 and CCND3 hijack immunoglobulin light-chain enhancers in cyclin D12 mantle cell lymphoma. Blood, 133(9), 940-951. https://doi.org/10.1182/blood-2018-07-862151

CCND2 and CCND3 hijack immunoglobulin light-chain enhancers in cyclin D12 mantle cell lymphoma. / Martín-Garcia, David; Navarro, Alba; Valdés-Mas, Rafael; Clot, Guillem; Gutiérrez-Abril, Jesús; Prieto, Miriam; Ribera-Cortada, Inmaculada; Woroniecka, Renata; Rymkiewicz, Grzegorz; Bens, Susanne; De Leval, Laurence; Rosenwald, Andreas; Ferry, Judith A.; Hsi, Eric D.; Fu, Kai; Delabie, Jan; Weisenburger, Dennis; De Jong, Daphne; Climent, Fina; O'Connor, Sheila J.; Swerdlow, Steven H.; Torrents, David; Beltran, Sergi; Espinet, Blanca; González-Farre, Blanca; Veloza, Luis; Costa, Dolors; Matutes, Estella; Siebert, Reiner; Ott, German; Quintanilla-Martinez, Leticia; Jaffe, Elaine S.; López-Otín, Carlos; Salaverria, Itziar; Puente, Xose S.; Campo, Elias; Bea, Silvia.

In: Blood, Vol. 133, No. 9, 01.01.2019, p. 940-951.

Research output: Contribution to journalArticle

Martín-Garcia, D, Navarro, A, Valdés-Mas, R, Clot, G, Gutiérrez-Abril, J, Prieto, M, Ribera-Cortada, I, Woroniecka, R, Rymkiewicz, G, Bens, S, De Leval, L, Rosenwald, A, Ferry, JA, Hsi, ED, Fu, K, Delabie, J, Weisenburger, D, De Jong, D, Climent, F, O'Connor, SJ, Swerdlow, SH, Torrents, D, Beltran, S, Espinet, B, González-Farre, B, Veloza, L, Costa, D, Matutes, E, Siebert, R, Ott, G, Quintanilla-Martinez, L, Jaffe, ES, López-Otín, C, Salaverria, I, Puente, XS, Campo, E & Bea, S 2019, 'CCND2 and CCND3 hijack immunoglobulin light-chain enhancers in cyclin D12 mantle cell lymphoma', Blood, vol. 133, no. 9, pp. 940-951. https://doi.org/10.1182/blood-2018-07-862151
Martín-Garcia D, Navarro A, Valdés-Mas R, Clot G, Gutiérrez-Abril J, Prieto M et al. CCND2 and CCND3 hijack immunoglobulin light-chain enhancers in cyclin D12 mantle cell lymphoma. Blood. 2019 Jan 1;133(9):940-951. https://doi.org/10.1182/blood-2018-07-862151
Martín-Garcia, David ; Navarro, Alba ; Valdés-Mas, Rafael ; Clot, Guillem ; Gutiérrez-Abril, Jesús ; Prieto, Miriam ; Ribera-Cortada, Inmaculada ; Woroniecka, Renata ; Rymkiewicz, Grzegorz ; Bens, Susanne ; De Leval, Laurence ; Rosenwald, Andreas ; Ferry, Judith A. ; Hsi, Eric D. ; Fu, Kai ; Delabie, Jan ; Weisenburger, Dennis ; De Jong, Daphne ; Climent, Fina ; O'Connor, Sheila J. ; Swerdlow, Steven H. ; Torrents, David ; Beltran, Sergi ; Espinet, Blanca ; González-Farre, Blanca ; Veloza, Luis ; Costa, Dolors ; Matutes, Estella ; Siebert, Reiner ; Ott, German ; Quintanilla-Martinez, Leticia ; Jaffe, Elaine S. ; López-Otín, Carlos ; Salaverria, Itziar ; Puente, Xose S. ; Campo, Elias ; Bea, Silvia. / CCND2 and CCND3 hijack immunoglobulin light-chain enhancers in cyclin D12 mantle cell lymphoma. In: Blood. 2019 ; Vol. 133, No. 9. pp. 940-951.
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title = "CCND2 and CCND3 hijack immunoglobulin light-chain enhancers in cyclin D12 mantle cell lymphoma",
abstract = "Mantle cell lymphoma (MCL) is characterized by the t(11;14)(q13;q32) translocation resulting in overexpression of cyclin D1. However, a small subset of cyclin D12 MCL has been recognized, and approximately one-half of them harbor CCND2 translocations while the primary event in cyclinD12/D22MCL remains elusive. To identify other potentialmechanisms driving MCL pathogenesis, we investigated 56 cyclin D12/SOX111 MCL by fluorescence in situ hybridization (FISH), whole-genome/exome sequencing, and gene-expression and copy-number arrays. FISH with break-apart probes identified CCND2 rearrangements in 39 cases (70{\%}) but not CCND3 rearrangements. We analyzed 3 of these negative cases by whole-genome/exome sequencing and identified IGK (n 5 2) and IGL (n 5 1) enhancer hijackings near CCND3 that were associated with cyclin D3 overexpression. By specific FISH probes, including the IGK enhancer region, we detected 10 additional cryptic IGK juxtapositions to CCND3 (6 cases) and CCND2 (4 cases) in MCL that overexpressed, respectively, these cyclins. A minor subset of 4 cyclin D12 MCL cases lacked cyclin D rearrangements and showed upregulation of CCNE1 and CCNE2. These cases had blastoid morphology, high genomic complexity, and CDKN2A and RB1 deletions. Both genomic and gene-expression profiles of cyclin D12 MCL cases were indistinguishable from cyclin D11 MCL. In conclusion, virtually all cyclin D12 MCLs carry CCND2/CCND3 rearrangements with immunoglobulin genes, including a novel IGK/L enhancer hijacking mechanism. A subset of cyclin D12/D22/D32 MCL with aggressive features has cyclin E dysregulation. Specific FISH probes may allow the molecular identification and diagnosis of cyclin D12 MCL.",
author = "David Mart{\'i}n-Garcia and Alba Navarro and Rafael Vald{\'e}s-Mas and Guillem Clot and Jes{\'u}s Guti{\'e}rrez-Abril and Miriam Prieto and Inmaculada Ribera-Cortada and Renata Woroniecka and Grzegorz Rymkiewicz and Susanne Bens and {De Leval}, Laurence and Andreas Rosenwald and Ferry, {Judith A.} and Hsi, {Eric D.} and Kai Fu and Jan Delabie and Dennis Weisenburger and {De Jong}, Daphne and Fina Climent and O'Connor, {Sheila J.} and Swerdlow, {Steven H.} and David Torrents and Sergi Beltran and Blanca Espinet and Blanca Gonz{\'a}lez-Farre and Luis Veloza and Dolors Costa and Estella Matutes and Reiner Siebert and German Ott and Leticia Quintanilla-Martinez and Jaffe, {Elaine S.} and Carlos L{\'o}pez-Ot{\'i}n and Itziar Salaverria and Puente, {Xose S.} and Elias Campo and Silvia Bea",
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T1 - CCND2 and CCND3 hijack immunoglobulin light-chain enhancers in cyclin D12 mantle cell lymphoma

AU - Martín-Garcia, David

AU - Navarro, Alba

AU - Valdés-Mas, Rafael

AU - Clot, Guillem

AU - Gutiérrez-Abril, Jesús

AU - Prieto, Miriam

AU - Ribera-Cortada, Inmaculada

AU - Woroniecka, Renata

AU - Rymkiewicz, Grzegorz

AU - Bens, Susanne

AU - De Leval, Laurence

AU - Rosenwald, Andreas

AU - Ferry, Judith A.

AU - Hsi, Eric D.

AU - Fu, Kai

AU - Delabie, Jan

AU - Weisenburger, Dennis

AU - De Jong, Daphne

AU - Climent, Fina

AU - O'Connor, Sheila J.

AU - Swerdlow, Steven H.

AU - Torrents, David

AU - Beltran, Sergi

AU - Espinet, Blanca

AU - González-Farre, Blanca

AU - Veloza, Luis

AU - Costa, Dolors

AU - Matutes, Estella

AU - Siebert, Reiner

AU - Ott, German

AU - Quintanilla-Martinez, Leticia

AU - Jaffe, Elaine S.

AU - López-Otín, Carlos

AU - Salaverria, Itziar

AU - Puente, Xose S.

AU - Campo, Elias

AU - Bea, Silvia

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Mantle cell lymphoma (MCL) is characterized by the t(11;14)(q13;q32) translocation resulting in overexpression of cyclin D1. However, a small subset of cyclin D12 MCL has been recognized, and approximately one-half of them harbor CCND2 translocations while the primary event in cyclinD12/D22MCL remains elusive. To identify other potentialmechanisms driving MCL pathogenesis, we investigated 56 cyclin D12/SOX111 MCL by fluorescence in situ hybridization (FISH), whole-genome/exome sequencing, and gene-expression and copy-number arrays. FISH with break-apart probes identified CCND2 rearrangements in 39 cases (70%) but not CCND3 rearrangements. We analyzed 3 of these negative cases by whole-genome/exome sequencing and identified IGK (n 5 2) and IGL (n 5 1) enhancer hijackings near CCND3 that were associated with cyclin D3 overexpression. By specific FISH probes, including the IGK enhancer region, we detected 10 additional cryptic IGK juxtapositions to CCND3 (6 cases) and CCND2 (4 cases) in MCL that overexpressed, respectively, these cyclins. A minor subset of 4 cyclin D12 MCL cases lacked cyclin D rearrangements and showed upregulation of CCNE1 and CCNE2. These cases had blastoid morphology, high genomic complexity, and CDKN2A and RB1 deletions. Both genomic and gene-expression profiles of cyclin D12 MCL cases were indistinguishable from cyclin D11 MCL. In conclusion, virtually all cyclin D12 MCLs carry CCND2/CCND3 rearrangements with immunoglobulin genes, including a novel IGK/L enhancer hijacking mechanism. A subset of cyclin D12/D22/D32 MCL with aggressive features has cyclin E dysregulation. Specific FISH probes may allow the molecular identification and diagnosis of cyclin D12 MCL.

AB - Mantle cell lymphoma (MCL) is characterized by the t(11;14)(q13;q32) translocation resulting in overexpression of cyclin D1. However, a small subset of cyclin D12 MCL has been recognized, and approximately one-half of them harbor CCND2 translocations while the primary event in cyclinD12/D22MCL remains elusive. To identify other potentialmechanisms driving MCL pathogenesis, we investigated 56 cyclin D12/SOX111 MCL by fluorescence in situ hybridization (FISH), whole-genome/exome sequencing, and gene-expression and copy-number arrays. FISH with break-apart probes identified CCND2 rearrangements in 39 cases (70%) but not CCND3 rearrangements. We analyzed 3 of these negative cases by whole-genome/exome sequencing and identified IGK (n 5 2) and IGL (n 5 1) enhancer hijackings near CCND3 that were associated with cyclin D3 overexpression. By specific FISH probes, including the IGK enhancer region, we detected 10 additional cryptic IGK juxtapositions to CCND3 (6 cases) and CCND2 (4 cases) in MCL that overexpressed, respectively, these cyclins. A minor subset of 4 cyclin D12 MCL cases lacked cyclin D rearrangements and showed upregulation of CCNE1 and CCNE2. These cases had blastoid morphology, high genomic complexity, and CDKN2A and RB1 deletions. Both genomic and gene-expression profiles of cyclin D12 MCL cases were indistinguishable from cyclin D11 MCL. In conclusion, virtually all cyclin D12 MCLs carry CCND2/CCND3 rearrangements with immunoglobulin genes, including a novel IGK/L enhancer hijacking mechanism. A subset of cyclin D12/D22/D32 MCL with aggressive features has cyclin E dysregulation. Specific FISH probes may allow the molecular identification and diagnosis of cyclin D12 MCL.

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