Cationic liposome-mediated incorporation of prostatic acid phosphatase protein into human prostate carcinoma cells

Ming Fong Lin, Julie DaVolio, Renee Garcia

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Lipofectin, the commercially available cationic liposome, was used to introduce the purified prostatic acid phosphatase protein into the established human prostate carcinoma cells. The incorporated phosphatase protein which retained its enzymatic activity as demonstrated by the tartrate-sensitive acid phosphatase assay was localized in the cytoplasm by immuriofluorescence staining. Further, cells that were treated with phosphatase/Lipofectin complexes expressed a decreased phosphotyrosine level, presumably due to the endogenous protein tyrosine phosphatase activity of the acid phosphatase protein. A cationic liposome such as lipofectin may thus be employed to mediate transport of other acidic proteins into cells, providing a way to examine their biological functions in vivo.

Original languageEnglish (US)
Pages (from-to)413-419
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume192
Issue number2
DOIs
StatePublished - Apr 30 1993

Fingerprint

Liposomes
Prostate
Cells
Acid Phosphatase
Carcinoma
Phosphotyrosine
Proteins
Protein Tyrosine Phosphatases
Phosphoprotein Phosphatases
Phosphoric Monoester Hydrolases
Assays
Cytoplasm
Staining and Labeling
1,2-dielaidoylphosphatidylethanolamine
prostatic acid phosphatase
tartrate-sensitive acid phosphatase
tartaric acid

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Cationic liposome-mediated incorporation of prostatic acid phosphatase protein into human prostate carcinoma cells. / Lin, Ming Fong; DaVolio, Julie; Garcia, Renee.

In: Biochemical and Biophysical Research Communications, Vol. 192, No. 2, 30.04.1993, p. 413-419.

Research output: Contribution to journalArticle

@article{0c8df4076c9a481aa9e9286b972ac8fa,
title = "Cationic liposome-mediated incorporation of prostatic acid phosphatase protein into human prostate carcinoma cells",
abstract = "Lipofectin, the commercially available cationic liposome, was used to introduce the purified prostatic acid phosphatase protein into the established human prostate carcinoma cells. The incorporated phosphatase protein which retained its enzymatic activity as demonstrated by the tartrate-sensitive acid phosphatase assay was localized in the cytoplasm by immuriofluorescence staining. Further, cells that were treated with phosphatase/Lipofectin complexes expressed a decreased phosphotyrosine level, presumably due to the endogenous protein tyrosine phosphatase activity of the acid phosphatase protein. A cationic liposome such as lipofectin may thus be employed to mediate transport of other acidic proteins into cells, providing a way to examine their biological functions in vivo.",
author = "Lin, {Ming Fong} and Julie DaVolio and Renee Garcia",
year = "1993",
month = "4",
day = "30",
doi = "10.1006/bbrc.1993.1431",
language = "English (US)",
volume = "192",
pages = "413--419",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Cationic liposome-mediated incorporation of prostatic acid phosphatase protein into human prostate carcinoma cells

AU - Lin, Ming Fong

AU - DaVolio, Julie

AU - Garcia, Renee

PY - 1993/4/30

Y1 - 1993/4/30

N2 - Lipofectin, the commercially available cationic liposome, was used to introduce the purified prostatic acid phosphatase protein into the established human prostate carcinoma cells. The incorporated phosphatase protein which retained its enzymatic activity as demonstrated by the tartrate-sensitive acid phosphatase assay was localized in the cytoplasm by immuriofluorescence staining. Further, cells that were treated with phosphatase/Lipofectin complexes expressed a decreased phosphotyrosine level, presumably due to the endogenous protein tyrosine phosphatase activity of the acid phosphatase protein. A cationic liposome such as lipofectin may thus be employed to mediate transport of other acidic proteins into cells, providing a way to examine their biological functions in vivo.

AB - Lipofectin, the commercially available cationic liposome, was used to introduce the purified prostatic acid phosphatase protein into the established human prostate carcinoma cells. The incorporated phosphatase protein which retained its enzymatic activity as demonstrated by the tartrate-sensitive acid phosphatase assay was localized in the cytoplasm by immuriofluorescence staining. Further, cells that were treated with phosphatase/Lipofectin complexes expressed a decreased phosphotyrosine level, presumably due to the endogenous protein tyrosine phosphatase activity of the acid phosphatase protein. A cationic liposome such as lipofectin may thus be employed to mediate transport of other acidic proteins into cells, providing a way to examine their biological functions in vivo.

UR - http://www.scopus.com/inward/record.url?scp=0027183359&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027183359&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1993.1431

DO - 10.1006/bbrc.1993.1431

M3 - Article

C2 - 7683459

AN - SCOPUS:0027183359

VL - 192

SP - 413

EP - 419

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -