Cationic lipid-based gene delivery systems

Pharmaceutical perspectives

Ram I Mahato, Alain Rolland, Eric Tomlinson

Research output: Contribution to journalReview article

195 Citations (Scopus)

Abstract

Gene delivery systems are designed to control the location of administered therapeutic genes within a patient's body. Successful in vivo gene transfer may require (i) the condensation of plasmid and its protection from nuclease degradation, (ii) cellular interaction and internalization of condensed plasmid, (iii) escape of plasmid from endosomes (if endocytosis is involved), and (iv) plasmid entry into cell nuclei. Expression plasmids encoding a therapeutic protein can be, for instance, complexed with cationic liposomes or micelles in order to achieve effective in vivo gene transfer. A thorough knowledge of pharmaceutics and drug delivery, bio-engineering, as well as cell and molecular biology is required to design optimal systems for gene therapy. This mini-review provides a critical discussion on cationic lipid-based gene delivery systems and their possible uses as pharmaceuticals.

Original languageEnglish (US)
Pages (from-to)853-859
Number of pages7
JournalPharmaceutical Research
Volume14
Issue number7
DOIs
StatePublished - Aug 19 1997

Fingerprint

Gene Transfer Techniques
Plasmids
Genes
Lipids
Gene transfer
Pharmaceutical Preparations
Cytology
Gene therapy
Bioengineering
Optimal systems
Molecular biology
Endosomes
Micelles
Endocytosis
Cell Nucleus
Drug delivery
Liposomes
Genetic Therapy
Cell Biology
Molecular Biology

Keywords

  • Cationic liposomes
  • Formulation
  • Non-viral gene delivery
  • Plasmid
  • Transfection

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry
  • Pharmacology (medical)

Cite this

Cationic lipid-based gene delivery systems : Pharmaceutical perspectives. / Mahato, Ram I; Rolland, Alain; Tomlinson, Eric.

In: Pharmaceutical Research, Vol. 14, No. 7, 19.08.1997, p. 853-859.

Research output: Contribution to journalReview article

Mahato, Ram I ; Rolland, Alain ; Tomlinson, Eric. / Cationic lipid-based gene delivery systems : Pharmaceutical perspectives. In: Pharmaceutical Research. 1997 ; Vol. 14, No. 7. pp. 853-859.
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