Caspase 1 activity influences juvenile Batten disease (CLN3) pathogenesis

Maria Burkovetskaya, Megan E. Bosch, Nikolay Karpuk, Rachel Fallet, Tammy L Kielian

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Juvenile Neuronal Ceroid Lipofuscinosis (JNCL) is an autosomal recessive lysosomal storage disease caused by loss-of-function mutations in CLN3. Symptoms appear between 5 and 10 years of age, beginning with blindness and seizures, followed by progressive cognitive and motor decline, and premature death. Glial activation and impaired neuronal activity are early signs of pathology in the Cln3Δex7/8 mouse model of JNCL, whereas neuron death occurs much later in the disease process. We previously reported that Cln3Δex7/8 microglia are primed toward a pro-inflammatory phenotype typified by exaggerated caspase 1 inflammasome activation and here we extend those findings to demonstrate heightened caspase activity in the Cln3Δex7/8 mouse brain. Based on the ability of caspase 1 to cleave a large number of substrates that have been implicated in JNCL pathology, we examined the functional implications of caspase 1 inflammasome activity by crossing Cln3Δex7/8 and caspase 1-deficient mice to create Cln3Δex7/8/Casp-1−/− animals. Caspase 1 deletion influenced motor behavior deficits and astrocyte activation in the context of CLN3 mutation, since both were significantly reversed in Cln3Δex7/8/Casp-1−/− mice, with phenotypes approaching that of wild-type animals. We also report a progressive age-dependent reduction in whisker length in Cln3Δex7/8 mice that was partially caspase 1-dependent. However, not all CLN3 phenotypes were reversed following caspase 1 deletion, since no significant differences in lysosomal accumulation or microglial activation were observed between Cln3Δex7/8 and Cln3Δex7/8/Casp-1−/− mice. Although the molecular targets of aberrant caspase 1 activity in the context of CLN3 mutation remain to be identified, our studies suggest that caspase 1 may represent a potential therapeutic target to mitigate some attributes of CLN3 disease. (Figure presented.). This article is part of the Special Issue “Lysosomal Storage Disorders”.

Original languageEnglish (US)
JournalJournal of Neurochemistry
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Neuronal Ceroid-Lipofuscinoses
Caspase 1
Ceroid
Chemical activation
Inflammasomes
Pathology
Phenotype
Mutation
Disease Attributes
Animals
Lysosomal Storage Diseases
Vibrissae
Premature Mortality
Aptitude
Wild Animals
Microglia
Blindness
Caspases
Neuroglia
Astrocytes

Keywords

  • Caspase 1
  • CLN3
  • juvenile Batten disease

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Caspase 1 activity influences juvenile Batten disease (CLN3) pathogenesis. / Burkovetskaya, Maria; Bosch, Megan E.; Karpuk, Nikolay; Fallet, Rachel; Kielian, Tammy L.

In: Journal of Neurochemistry, 01.01.2018.

Research output: Contribution to journalArticle

Burkovetskaya, Maria ; Bosch, Megan E. ; Karpuk, Nikolay ; Fallet, Rachel ; Kielian, Tammy L. / Caspase 1 activity influences juvenile Batten disease (CLN3) pathogenesis. In: Journal of Neurochemistry. 2018.
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abstract = "Juvenile Neuronal Ceroid Lipofuscinosis (JNCL) is an autosomal recessive lysosomal storage disease caused by loss-of-function mutations in CLN3. Symptoms appear between 5 and 10 years of age, beginning with blindness and seizures, followed by progressive cognitive and motor decline, and premature death. Glial activation and impaired neuronal activity are early signs of pathology in the Cln3Δex7/8 mouse model of JNCL, whereas neuron death occurs much later in the disease process. We previously reported that Cln3Δex7/8 microglia are primed toward a pro-inflammatory phenotype typified by exaggerated caspase 1 inflammasome activation and here we extend those findings to demonstrate heightened caspase activity in the Cln3Δex7/8 mouse brain. Based on the ability of caspase 1 to cleave a large number of substrates that have been implicated in JNCL pathology, we examined the functional implications of caspase 1 inflammasome activity by crossing Cln3Δex7/8 and caspase 1-deficient mice to create Cln3Δex7/8/Casp-1−/− animals. Caspase 1 deletion influenced motor behavior deficits and astrocyte activation in the context of CLN3 mutation, since both were significantly reversed in Cln3Δex7/8/Casp-1−/− mice, with phenotypes approaching that of wild-type animals. We also report a progressive age-dependent reduction in whisker length in Cln3Δex7/8 mice that was partially caspase 1-dependent. However, not all CLN3 phenotypes were reversed following caspase 1 deletion, since no significant differences in lysosomal accumulation or microglial activation were observed between Cln3Δex7/8 and Cln3Δex7/8/Casp-1−/− mice. Although the molecular targets of aberrant caspase 1 activity in the context of CLN3 mutation remain to be identified, our studies suggest that caspase 1 may represent a potential therapeutic target to mitigate some attributes of CLN3 disease. (Figure presented.). This article is part of the Special Issue “Lysosomal Storage Disorders”.",
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