Cardiac stem cell niche, mmp9, and culture and differentiation of embryonic stem cells

Paras Kumar Mishra, Nicholas John Kuypers, Shree Ram Singh, Noel Diaz Leiberh, Vishalakshi Chavali, Suresh C. Tyagi

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

Embryonic stem cells (ESC) are totipotent, self-renewing, and clonogenic, having potential to differentiate into a wide variety of cell types. Due to regenerative capability, it has tremendous potential for treating myocardial infarction (death of myocardial tissue) and type 1 diabetes (death of pancreatic beta cells). Understanding the components regulating ESC differentiation is the key to unlock the regenerative potential of ESC-based therapies. Both the stiffness of extracellular matrix (ECM) and surrounding niche/microenvironment play pivotal roles in ESC differentiation. Matrix metalloproteinase-9 (MMP9) induces fi brosis that causes stiffness of the ECM and impairs differentiation of cardiac stem cells into cardiomyocytes. Here, we describe the method of ESC culture and differentiation, and the expression of MMP9 and its inhibitor, tissue inhibitor of metalloproteinase-4 (TIMP4) in differentiating ESC.

Original languageEnglish (US)
Title of host publicationStem Cell Niche
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages153-163
Number of pages11
ISBN (Print)9781627035071
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1035
ISSN (Print)1064-3745

Fingerprint

Stem Cell Niche
Embryonic Stem Cells
Cell Differentiation
Matrix Metalloproteinase 9
Extracellular Matrix
Insulin-Secreting Cells
Cell- and Tissue-Based Therapy
Type 1 Diabetes Mellitus
Cardiac Myocytes
Stem Cells
Cell Culture Techniques
Myocardial Infarction

Keywords

  • Cardiomyocytes
  • Differentiation
  • Extracellular matrix
  • MMP9
  • Stem cell
  • TIMP-4

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Mishra, P. K., Kuypers, N. J., Singh, S. R., Leiberh, N. D., Chavali, V., & Tyagi, S. C. (2013). Cardiac stem cell niche, mmp9, and culture and differentiation of embryonic stem cells. In Stem Cell Niche: Methods and Protocols (pp. 153-163). (Methods in Molecular Biology; Vol. 1035). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-508-8_13

Cardiac stem cell niche, mmp9, and culture and differentiation of embryonic stem cells. / Mishra, Paras Kumar; Kuypers, Nicholas John; Singh, Shree Ram; Leiberh, Noel Diaz; Chavali, Vishalakshi; Tyagi, Suresh C.

Stem Cell Niche: Methods and Protocols. Humana Press Inc., 2013. p. 153-163 (Methods in Molecular Biology; Vol. 1035).

Research output: Chapter in Book/Report/Conference proceedingChapter

Mishra, PK, Kuypers, NJ, Singh, SR, Leiberh, ND, Chavali, V & Tyagi, SC 2013, Cardiac stem cell niche, mmp9, and culture and differentiation of embryonic stem cells. in Stem Cell Niche: Methods and Protocols. Methods in Molecular Biology, vol. 1035, Humana Press Inc., pp. 153-163. https://doi.org/10.1007/978-1-62703-508-8_13
Mishra PK, Kuypers NJ, Singh SR, Leiberh ND, Chavali V, Tyagi SC. Cardiac stem cell niche, mmp9, and culture and differentiation of embryonic stem cells. In Stem Cell Niche: Methods and Protocols. Humana Press Inc. 2013. p. 153-163. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-508-8_13
Mishra, Paras Kumar ; Kuypers, Nicholas John ; Singh, Shree Ram ; Leiberh, Noel Diaz ; Chavali, Vishalakshi ; Tyagi, Suresh C. / Cardiac stem cell niche, mmp9, and culture and differentiation of embryonic stem cells. Stem Cell Niche: Methods and Protocols. Humana Press Inc., 2013. pp. 153-163 (Methods in Molecular Biology).
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