Cardiac fibronectin

Developmental distribution and quantitative comparison of possible sites of synthesis

Gail G. Ahumada, Stephen Israel Rennard, Alvaro A. Figueroa, Michael H. Silver

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Evidence accumulating from other cell systems suggests that fibronectin, if present in the heart, might modulate patterns of cell organization during myocardial development. Accordingly, we examined hearts of fetal, neonatal and adult rats for the presence and distribution of fibronectin, and quantitatively measured fibronectin synthesis by cultured cardiac myocytes and mesenchymal cells to assess the relative importance of these potential intracardiac sources of this glycoprotein. Fibronectin was present in the hearts from all age groups and was distributed in a reticular pattern suggesting a pericellular or interstitial location. In fetal hearts, specific immuno-fluorescence was particularly prominent at the crest of the developing interventricular septum. In post-confluent cardiac mesenchymal cultures, a dense fibrillar network of fibronectin formed over the surface of the cells. The content of fibronectin in the culture medium assessed by enzyme-linked immunosorbent assay increased substantially at confluence from 2 to 17 mg/dish, stabilizing at 11 mg in post-confluent mesenchymal cultures. In myocytic cultures, fibronectin formed a fine reticular pattern over cell surfaces and the fibronectin content of the medium ranged from 0.8 to 2.1 mg. Thus, fibronectin is present in the heart, may be synthesized in the neonate predominantly by cardiac mesenchymal cells, and may contribute by mechanisms still to be characterized in vivo to normal development of the critical septal region during embryogenesis.

Original languageEnglish (US)
Pages (from-to)667-678
Number of pages12
JournalJournal of Molecular and Cellular Cardiology
Volume13
Issue number7
DOIs
StatePublished - Jan 1 1981

Fingerprint

Fibronectins
Fetal Heart
Septum of Brain
Cardiac Myocytes
Embryonic Development
Culture Media
Glycoproteins
Age Groups
Fluorescence
Enzyme-Linked Immunosorbent Assay
Newborn Infant

Keywords

  • Cardiac glycoproteins
  • Fibronectin in rat heart
  • Myocardial development

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Cardiac fibronectin : Developmental distribution and quantitative comparison of possible sites of synthesis. / Ahumada, Gail G.; Rennard, Stephen Israel; Figueroa, Alvaro A.; Silver, Michael H.

In: Journal of Molecular and Cellular Cardiology, Vol. 13, No. 7, 01.01.1981, p. 667-678.

Research output: Contribution to journalArticle

Ahumada, Gail G. ; Rennard, Stephen Israel ; Figueroa, Alvaro A. ; Silver, Michael H. / Cardiac fibronectin : Developmental distribution and quantitative comparison of possible sites of synthesis. In: Journal of Molecular and Cellular Cardiology. 1981 ; Vol. 13, No. 7. pp. 667-678.
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