Cardiac extracellular proteome profiling and membrane topology analysis using glycoproteomics

Yuan Tian, Tejaswi Koganti, Zhihao Yao, Presley Cannon, Punit Shah, Laura Pietrovito, Alessandra Modesti, Paul Aiyetan, Kristine Deleon-Pennell, Yonggang Ma, Ganesh V. Halade, Chindo Hicks, Hui Zhang, Merry L Lindsey

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Purpose: Extracellular proteins are easily accessible, which presents a subproteome of molecular targets that have high diagnostic and therapeutic potential. Efforts have been made to catalog the cardiac extracellular matridome and analyze the topology of identified proteins for the design of therapeutic targets. Although many bioinformatics tools have been developed to predict protein topology, topology has been experimentally validated for only a very small portion of membrane proteins. The aim of this study was to use a glycoproteomics and MS approach to identify glycoproteins in the extracellular matridome of the infarcted left ventricle (LV) and provide experimental evidence for topological determination. Experimental design: Glycoproteomics analysis was performed on eight biological replicates of LV samples from wild-type mice at 7 days following myocardial infarction using SPE of glycopeptides, followed by mass spectrometric identification of N-linked glycosylation sites for topology assessment. Results: We identified hundreds of glycoproteins, and the identified N-glycosylation sites provide novel information on the correct topology for membrane proteins present in the infarct setting. Conclusions and clinical relevance: Our data provide the foundation for future studies of the LV infarct extracellular matridome, which may facilitate the discovery of drug targets and biomarkers.

Original languageEnglish (US)
Pages (from-to)595-602
Number of pages8
JournalProteomics - Clinical Applications
Volume8
Issue number7-8
DOIs
StatePublished - Jan 1 2014

Fingerprint

Proteome
Heart Ventricles
Topology
Membranes
Glycosylation
Glycoproteins
Membrane Proteins
Proteins
Glycopeptides
Drug Discovery
Computational Biology
Research Design
Biomarkers
Myocardial Infarction
Bioinformatics
Design of experiments
Therapeutics
Pharmaceutical Preparations

Keywords

  • Extracellular matridome
  • Glycoprotein
  • Left ventricle
  • Matrix metalloproteinase
  • Membrane orientation
  • Myocardial infarction

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Cardiac extracellular proteome profiling and membrane topology analysis using glycoproteomics. / Tian, Yuan; Koganti, Tejaswi; Yao, Zhihao; Cannon, Presley; Shah, Punit; Pietrovito, Laura; Modesti, Alessandra; Aiyetan, Paul; Deleon-Pennell, Kristine; Ma, Yonggang; Halade, Ganesh V.; Hicks, Chindo; Zhang, Hui; Lindsey, Merry L.

In: Proteomics - Clinical Applications, Vol. 8, No. 7-8, 01.01.2014, p. 595-602.

Research output: Contribution to journalArticle

Tian, Y, Koganti, T, Yao, Z, Cannon, P, Shah, P, Pietrovito, L, Modesti, A, Aiyetan, P, Deleon-Pennell, K, Ma, Y, Halade, GV, Hicks, C, Zhang, H & Lindsey, ML 2014, 'Cardiac extracellular proteome profiling and membrane topology analysis using glycoproteomics', Proteomics - Clinical Applications, vol. 8, no. 7-8, pp. 595-602. https://doi.org/10.1002/prca.201400009
Tian, Yuan ; Koganti, Tejaswi ; Yao, Zhihao ; Cannon, Presley ; Shah, Punit ; Pietrovito, Laura ; Modesti, Alessandra ; Aiyetan, Paul ; Deleon-Pennell, Kristine ; Ma, Yonggang ; Halade, Ganesh V. ; Hicks, Chindo ; Zhang, Hui ; Lindsey, Merry L. / Cardiac extracellular proteome profiling and membrane topology analysis using glycoproteomics. In: Proteomics - Clinical Applications. 2014 ; Vol. 8, No. 7-8. pp. 595-602.
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