Carboxyl terminus of plakophilin-1 recruits it to plasma membrane, whereas amino terminus recruits desmoplakin and promotes desmosome assembly

Tammy Sobolik-Delmaire, Dawn Katafiasz, James K Wahl

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Plakophilins are armadillo repeat-containing proteins, initially identified as desmosomal plaque proteins that have subsequently been shown to also localize to the nucleus. Loss of plakophilin-1 is the underlying cause of ectodermal dysplasia/skin fragility syndrome, and skin from these patients exhibits desmosomes that are reduced in size and number. Thus, it has been suggested that plakophilin-1 plays an important role in desmosome stability and/or assembly. In this study, we used a cell culture system (A431DE cells) that expresses all of the proteins necessary to assemble a desmosome, except plakophilin-1. Using this cell line, we sought to determine the role of plakophilin-1 in de novo desmosome assembly. Whenexogenous plakophilin-1 was expressed in these cells, desmosomes were assembled, as assessed by electron microscopy and immunofluorescence localization of desmoplakin, into punctate structures. Deletion mutagenesis experiments revealed that amino acids 686-726 in the carboxyl terminus of plakophilin-1 are required for its localization to the plasmamembrane. In addition,weshowed that amino acids 1-34 in the amino terminus were necessary for subsequent recruitment of desmoplakin to the membrane and desmosome assembly.

Original languageEnglish (US)
Pages (from-to)16962-16970
Number of pages9
JournalJournal of Biological Chemistry
Volume281
Issue number25
DOIs
StatePublished - Jun 23 2006

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Plakophilins
Desmoplakins
Desmosomes
Cell membranes
Cell Membrane
Armadillo Domain Proteins
Skin
Amino Acids
Mutagenesis
Cell culture
Electron microscopy
Fluorescent Antibody Technique
Electron Microscopy
Proteins
Cell Culture Techniques
Cells
Membranes
Cell Line

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Carboxyl terminus of plakophilin-1 recruits it to plasma membrane, whereas amino terminus recruits desmoplakin and promotes desmosome assembly. / Sobolik-Delmaire, Tammy; Katafiasz, Dawn; Wahl, James K.

In: Journal of Biological Chemistry, Vol. 281, No. 25, 23.06.2006, p. 16962-16970.

Research output: Contribution to journalArticle

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