Carbonic anhydrase in human pancreas

Hypotheses for the pathophysiological roles of CA isozymes

Isao Nishimori, Kiyomi Fujikawa-Adachi, Saburo Onishi, Michael A Hollingsworth

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Among more than ten isozymes of the carbonic anhydrase (CA) family, only cytoplasmic CA II and membrane bound CA IX have been reported to be expressed in human pancreas. To study the mRNA expression of CA isozymes in human pancreas, reverse transcriptase-polymerase chain reaction (RT-PCR)-Southern blot analysis and cDNA sequencing following RT-PCR were employed. CA II, IV, VI, IX, and XII were clearly identified in polyA+ RNA from normal human pancreas by RT-PCR-Southern blotting. Results with cultured pancreatic tumor cell lines suggest that CA II, IV, IX, and XII are expressed in the ductal cells, and CA VI is expressed in the acinar cells. We propose a hypothesis for the pathophysiological function of CA isozymes in human pancreas; (1) the intraluminal CA isozymes (CA IV, VI, and possibly XII) form a mutually complementary system with cytoplasmic CA II to regulate the luminal pH of the pancreatic duct system and work as a self defense mechanism against pancreatitis; (2) CA II and other CA isozymes play a pathological role in the autoimmune process of idiopathic chronic pancreatitis.

Original languageEnglish (US)
Pages (from-to)5-16
Number of pages12
JournalAnnals of the New York Academy of Sciences
Volume880
DOIs
StatePublished - Jan 1 1999

Fingerprint

Carbonic Anhydrase II
Carbonic Anhydrases
Isoenzymes
Pancreas
Carbonic Anhydrase IV
Reverse Transcriptase Polymerase Chain Reaction
Polymerase chain reaction
RNA-Directed DNA Polymerase
Southern Blotting
Cultured Tumor Cells
Acinar Cells
Pancreatic Ducts
Chronic Pancreatitis
Tumor Cell Line
Pancreatitis
Ducts
Complementary DNA
Tumors
RNA
Messenger RNA

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Carbonic anhydrase in human pancreas : Hypotheses for the pathophysiological roles of CA isozymes. / Nishimori, Isao; Fujikawa-Adachi, Kiyomi; Onishi, Saburo; Hollingsworth, Michael A.

In: Annals of the New York Academy of Sciences, Vol. 880, 01.01.1999, p. 5-16.

Research output: Contribution to journalArticle

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