Capillary electrophoresis-based immunoassays

Principles and quantitative applications

Research output: Contribution to journalReview article

41 Citations (Scopus)

Abstract

The use of CE as a tool to conduct immunoassays has been an area of increasing interest over the last decade. This approach combines the efficiency, small sample requirements, and relatively high speed of CE with the selectivity of antibodies as binding agents. This review examines the various assay formats and detection modes that have been reported for these assays, along with some representative applications. Most CE immunoassays in the past have employed homogeneous methods in which the sample and reagents are allowed to react in solution. These homogeneous methods have been conducted as both competitive binding immunoassays and as noncompetitive binding immunoassays. Fluorescent labels are most commonly used for detection in these assays, but enzyme labels have also been utilized for such work. Some additional work has been performed in CE immunoassays with heterogeneous methods in which either antibodies or an analog of the analyte is immobilized to a solid support. These heterogeneous methods can be used for the selective isolation of analytes prior to their separation by CE or to remove a given species from a sample/reagent mixture prior to analysis by CE. These CE immunoassays can be used with a variety of detection modes, such as fluorescence, UV/Vis absorbance, chemiluminescence, electrochemical measurements, MS, and surface plasmon resonance.

Original languageEnglish (US)
Pages (from-to)3279-3295
Number of pages17
JournalELECTROPHORESIS
Volume29
Issue number16 SPEC. ISS.
DOIs
StatePublished - Aug 1 2008

Fingerprint

Capillary electrophoresis
Capillary Electrophoresis
Immunoassay
Assays
Labels
Chemiluminescence
Antibodies
Surface plasmon resonance
Fluorescence
Luminescent Measurements
Competitive Binding
Surface Plasmon Resonance
Enzyme Assays
Enzymes

Keywords

  • Affinity capillary electrophoresis
  • Capillary electrophoresis
  • Capillary electrophoresis immunoassay
  • Immunoassay
  • Review

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Capillary electrophoresis-based immunoassays : Principles and quantitative applications. / Moser, Annette C; Hage, David S.

In: ELECTROPHORESIS, Vol. 29, No. 16 SPEC. ISS., 01.08.2008, p. 3279-3295.

Research output: Contribution to journalReview article

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